LRP1/CD91 Antibody - #DF2935

Fig. 2| (A) Characterization of LRP-1 receptor expressions on Caco-2 cells by CLSM.

Fig 1. The dynamic changes of mouse proteins in serum-derived exosomes in response to E. multilocularis infection. (A) and (B) Transmission electron microscopy of the enriched extracellular vesicles (EVs) isolated from the sera of E. multilocularis-infected (A) and uninfected mice (B). (C) The diameter distribution of the purified EVs. (D) Western blotting analysis of mammalian EV-marker CD63 in the purified EVs. (E) Heatmap of differentially abundance proteins between the serum exosomes from E. multilocularis-infected and uninfected mice. (F) Western blotting analysis the abundance of TGF-β1, GPATC8, LRP1, and AMY1 during E. multilocularis infection. https://doi.org/10.1371/journal.pntd.0010814.g001

Figure 2. |Evaluation of candidate gene/protein expression in GC cells. A, RT-qPCR detection of candidate gene expression in GC cells. Independent experiments were performed during the whole in vitro study. B, Western blot analysis of candidate protein expression levels in GC cells.

Figure 5. LRP1 is highly expressed in CRC patients with lymphatic invasion and high APOE level, and APOE-overexpression upregulates LRP1 protein level. (A) The intracellular and extracellular apoE levels of HCT116 and HCT8 cells with APOE-overexpression were determined by ELISA; (B) Schematic diagram of protein interactions of apoE. Data were obtained from the STRING database; (C) Comparison of LRP1 mRNA levels between the lymphatic invasion group and the non-lymphatic invasion group; (D) Comparison of LRP1 mRNA levels between the APOEHigh group (median APOE mRNA level > 10.24) and APOELow group (median APOE mRNA level < 10.24). Data were obtained from the UCSC Xena database; (E) Relative LRP1 mRNA levels of CRC cells with or without APOE-overexpression; (F) Determination of LRP1 protein levels in HCT116 and HCT8 cells with or without APOE-overexpression using western blot. apoA1: Apolipoprotein A1; apoA2: Apolipoprotein A2; apoB: Apolipoprotein B; apoC1: Apolipoprotein C1; apoC2: Apolipoprotein C2; apoC3: Apolipoprotein C3; apoE: Apolipoprotein E; APP: Amyloid precursor protein; CRC: Colorectal cancer; LDLR: Low-density lipoprotein receptor; LRP1: Lipoprotein receptor-related protein 1; LRP8: Lipoprotein receptor-related protein 8; OE: Overexpression; VC: Vector control; ns: No significance; ELISA: Enzyme-linked immunosorbent assay.

Figure 7. |The levels of efflux and reuptake proteins of cholesterol in CUMS rats. (A) The expression of ABCA1, LRP1, and ApoE in control and CUMS rats in the sixth week of the CUMS period.