Id1 Antibody - #DF2932

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产品: Id1 抗体
货号: DF2932
描述: Rabbit polyclonal antibody to Id1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit
蛋白号: P41134
RRID: AB_2840918

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Id1 Antibody detects endogenous levels of total Id1.
RRID:
AB_2840918
引用格式: Affinity Biosciences Cat# DF2932, RRID:AB_2840918.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLHb24; Class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); DNA binding protein inhibitor ID 1; DNA binding protein inhibitor ID1; DNA-binding protein inhibitor ID-1; Dominant negative helix loop helix protein; ID 1; ID; ID1; ID1_HUMAN; Inhibitor of Differentiation 1; Inhibitor of DNA binding 1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human Id1, corresponding to a region within the internal amino acids.

基因/基因ID:
ID1(3397)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

文献引用

1). Combined targeting of glioblastoma stem cells of different cellular states disrupts malignant progression. Nature communications, 2025 (PubMed: 40140646) [IF=16.6]

Application: WB    Species: Mouse    Sample:

Fig. 2: MEOX2 and SRGN are essential for GSCs proliferation, self-renewal, and stemness in CL and MES GSCs respectively. a, b Cell viability of indicated GSCs with shCONT, shMEOX2, or shSRGN measured by CellTiter-Glo assay (n = 6 per group). Data are presented as mean ± SD. c, e The number of spheres formed by CL and MES GSCs transduced with shCONT or shRNAs targeting MEOX2 or SRGN was quantified (n = 6 per group). Data are presented as mean ± SD. Box plots show median (center line), 25th–75th percentiles (box bounds), and whiskers extend to minimum and maximum values within 1.5 times the interquartile range. ns: non-significant. d, f In vitro limited dilution assay of CL and MES GSCs transduced with shCONT, shMEOX2-1/2 or shSRGN-1/2, respectively (n = 30 per group). χ2 test. g, j Heatmap of SOX2 and ID1 expression in MEOX2 and SRGN knockout RNA-seq data. h, k Quantitative RT-PCR assessment of SOX2 and ID1 mRNA levels in CL GSCs (3028, RKI) and MES GSCs (2907, 839) expressing shCONT, shMEOX2-1/2 or shSRGN-1/2, respectively. Data are presented as mean ± SD. n = 3 per group. i, l IB analysis of SOX2 and ID1 levels in CL GSCs (3028, RKI) and MES GSCs (2907, 839) transduced with shMEOX2, shSRGN, or control shRNA, respectively. m, o Bioluminescence imaging and quantification of orthotopic xenografts derived from luciferase-expressing 3028 (CL) and 2907 (2907) GSCs transduced with shCONT, shMEOX2, or shSRGN. Data are presented as mean ± SD. n = 3 per group. n, p Kaplan–Meier survival curves of mice bearing intracranial tumors derived from 3028 (CL) and 2907 (MES) GSCs (n = 6 per group) from the respective groups above. Statistics: a, b, c, e, m, o one-way ANOVA with Dunnett’s multiple-comparison test. d, f χ2 test for two-group comparison. h, k Unpaired Student’s t test for two-group comparison. n, p Log-rank test. Images: i, l Representative blots (n = 3). Source data are provided as a Source Data file.
2). Alpha-1 antitrypsin protects against phosgene-induced acute lung injury by activating the ID1-dependent anti-inflammatory response. European journal of pharmacology, 2023 (PubMed: 37673367) [IF=4.2]
3). Construction and experimental validation of a macrophage cell senescence-related gene signature to evaluate the prognosis, immunotherapeutic sensitivity, and chemotherapy response in bladder cancer. Functional & Integrative Genomics, 2023 (PubMed: 37423913) [IF=3.9]

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