Cofilin Antibody - #AF0761

Fig. 3 MiR-153-3p regulated breast cancer cell processes by binding to CFL2. A Dual-luciferase reporter assay was performed to demonstrate that miR-153-3p was directly associated with CFL2 in MCF-10AT and MCF-7 cells. B and C The protein level of CFL2 was detected by western blot in 42 pairs of breast cancer and paracancerous normal breast tissues as well as MCF-10A, MCF-10AT and MCF-7 cells. D–I MCF-10AT cells were transfected with in-miRNC, in-miR-153-3p, in-miR-153-3p+si-NC and in-miR-153-3p+si CFL2, respectively. D Cell viability was determined by CCK-8 assay. E Cell colony-forming ability was detected by cell colony-formation assay. F Cell apoptosis was analyzed by fow cytometry analysis. G and H Cell migratory and invasive capacities were determined by transwell assay. I The protein levels of E-cadherin and N-cadherin were determined by western blot analysis. A: adjacent normal breast tissues. T: breast cancer tissues. **P<0.01 and ***P<0.001