ITGAM Antibody - #DF2911

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产品: ITGAM 抗体
货号: DF2911
描述: Rabbit polyclonal antibody to ITGAM
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 170 kDa; 127kD(Calculated).
蛋白号: P11215
RRID: AB_2840900

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(88%), Horse(86%), Sheep(88%), Rabbit(88%), Dog(88%)
克隆:
Polyclonal
特异性:
ITGAM Antibody detects endogenous levels of total ITGAM.
RRID:
AB_2840900
引用格式: Affinity Biosciences Cat# DF2911, RRID:AB_2840900.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

antigen CD11b (p170); Antigen CD11b p170; CD11 antigen like family member B; CD11 antigen-like family member B; CD11b; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; Complement Component Receptor 3 Alpha; Complement receptor type 3, alpha subunit; CR 3 alpha chain (CR3A); CR 3 alpha chain; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; Integrin Alpha M; Integrin alpha M chain; Integrin alpha-M; Integrin beta 2 alpha subunit; Integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM_HUMAN; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; MAC 1; Mac-1a; MAC1; Mac1, alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MGC117044; Mo1, alpha subunit; MO1A; Neutrophil adherence receptor alpha M subunit; Neutrophil adherence receptor; SLEB6;

抗原和靶标

免疫原:
Uniprot:

P11215(ITAM_HUMAN) >>Visit HPA database.

基因/基因ID:
ITGAM(3684)
表达:
P11215 ITAM_HUMAN:

Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).

序列:
MALRVLLLTALTLCHGFNLDTENAMTFQENARGFGQSVVQLQGSRVVVGAPQEIVAANQRGSLYQCDYSTGSCEPIRLQVPVEAVNMSLGLSLAATTSPPQLLACGPTVHQTCSENTYVKGLCFLFGSNLRQQPQKFPEALRGCPQEDSDIAFLIDGSGSIIPHDFRRMKEFVSTVMEQLKKSKTLFSLMQYSEEFRIHFTFKEFQNNPNPRSLVKPITQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKPPRDHVFQVNNFEALKTIQNQLREKIFAIEGTQTGSSSSFEHEMSQEGFSAAITSNGPLLSTVGSYDWAGGVFLYTSKEKSTFINMTRVDSDMNDAYLGYAAAIILRNRVQSLVLGAPRYQHIGLVAMFRQNTGMWESNANVKGTQIGAYFGASLCSVDVDSNGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGRARWQCDAVLYGEQGQPWGRFGAALTVLGDVNGDKLTDVAIGAPGEEDNRGAVYLFHGTSGSGISPSHSQRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAGEVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVGTPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYRKVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLELPVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSDFLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEPFEVPNPLPLIVGSSVGGLLLLALITAALYKLGFFKRQYKDMMSEGGPPGAEPQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Horse
86
Pig
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11215 作为底物

Site PTM Type Enzyme
S574 Phosphorylation
S577 Phosphorylation
Y676 Phosphorylation
T805 Phosphorylation
N900 N-Glycosylation
T930 Phosphorylation
S931 Phosphorylation
N940 N-Glycosylation
N946 N-Glycosylation
S1142 Phosphorylation

研究背景

功能:

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).

细胞定位:

Cell membrane>Single-pass type I membrane protein. Membrane raft>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).

亚基结构:

Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18. Interacts with JAM3. Interacts with THBD. Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance.

蛋白家族:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION (PubMed: 35411569) [IF=8.5]

Application: IF/ICC    Species: rat    Sample: Dental pulp fibroblasts

FIGURE 5|Therapeutic effect of combined administration of RvE1 and LXA4 in a rat pulpitis model. (A) Representative haematoxylin–eosinstained images on days 1, 3 and 7 after surgery. (B) Representative immunofluorescence images of CD11b on days 1, 3 and 7 after surgery.
2). Comprehensive Analysis of HHLA2 as a Prognostic Biomarker and Its Association With Immune Infiltrates in Hepatocellular Carcinoma. Frontiers in Immunology (PubMed: 35371079) [IF=7.3]
3). Focal ischemic stroke modifies microglia-derived exosomal miRNAs: potential role of mir-212-5p in neuronal protection and functional recovery. Biological Research (PubMed: 37789455) [IF=6.7]

Application: WB    Species: Rat    Sample:

Fig. 2 MiRNA sequencing of microglial exosomes 3 days after MCAO/R and qRT–PCR validation. A, B Characterisation of microglial exosomes using nanoparticle tracking analysis and transmission electron microscopy scanning. Scale bar = 200 nm. C The exosome markers CD9, CD63, and CD81 were detected using western blot analysis. D Microglia-derived exosomes were detected by western blot analysis using the microglia marker CD11b. E Heatmap showing the levels of miRNAs in microglial exosomes at 3 days after MCAO/R. F Expression of miR-30c-5p, miR-126a-5p, miR-128-3p, miR-212-5p and miR-1949 in the ischemic penumbra of the cortex at 3 days after MCAO/R was determined using qRT–PCR. G Expression levels of the miRNA-targeting genes PLXNA2, PTEN and FOXO3 in the ischemic penumbra of the cortex at 3 days after MCAO/R. H The target sites of miR-212-5p in PLXNA2 mRNA 3’ untranslated region (3’UTR). I Map of the pmirGLO luciferase reporter vector. J Dual luciferase assays revealed the binding of miR-212-5p to the 3’UTR of PLXNA2. The data are presented as the means ± SEM (n = 5 per group). *P 
4). Inhibition of NLRP3 inflammasome activation and pyroptosis with the ethyl acetate fraction of Bungeanum ameliorated cognitive dysfunction in aged mice. Food & Function (PubMed: 34231604) [IF=6.1]
5). The Role of Bone Morphogenetic Protein 4 in Microglial Polarization in the Process of Neuropathic Pain. Journal of Inflammation Research (PubMed: 35535051) [IF=4.5]
6). Infiltration of Blood-Derived Macrophages Contributes to the Development of Diabetic Neuropathy. Journal of Immunology Research (PubMed: 31534976) [IF=4.1]
7). Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia. Molecular Medicine Reports (PubMed: 28393255) [IF=3.4]

Application: WB    Species: rat    Sample:

Figure 3. Western blot analysis of GFAP, Iba‑1, CD11b and SP‑A in the rat CNS at different stages of EAE. (A) Proteins obtained from the rat CNS at the initial, peak and recovery stages of EAE were detected for GFAP, IBA‑1, CD11b and SP‑A via western blotting. (B) Quantitative analysis of western blots was performed and levels were normalized relative to β‑actin. Results are presented as the mean ± standard deviation. * P

Application: IHC    Species: rat    Sample:

Figure 2. H&E staining and immunohistochemical staining of GFAP, Iba‑1, CD11b and SP‑A in the rat CNS at different stages of EAE. (A) CNS sections at initial, peak and recovery stages of EAE were stained for inflammation by H&E, GFAP‑positive astrocytes, Iba‑1‑postive microglia, CD11b general marker of inflammatory cells and SP‑A. Whole image magnification, x200 and corner image magnification, x400. Section of rats under normal conditions served as the control. Arrows indicate the location of SP‑A expressing cells. (B) Lu sections of normal rats were stained as a positive control. Negative control sections of normal rat CNS were treated with secondary antibody only and were unstained for GFAP, Iba‑1, CD11b and SP‑A. Magnification, x400. H&E, hematoxylin and eosin; GFAP, glial fibrillary acidic protein; Iba‑1, ionized calcium‑binding adapter molecule 1; SP‑A, surfactant protein‑A; Lu, lung; CNS, central nervous system; EAE, experimental autoimmune encephalomyelitis.
8). Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/miR-140-3p/TLR4 axis. European Journal of Histochemistry (PubMed: 37491974) [IF=2.0]

Application: WB    Species: Mouse    Sample: BV2 cells

Figure 5. Dex attenuates LPS-induced microglia activation by modulating circ-Shank3/miR-140-3p axis. BV2 cells were treated with 20 μg/mL Dex plus miR-140-3p mimics, circ-Shank3 siRNA1 or circ-Shank3 siRNA1 + miR-140-3p inhibitor, followed by treatment with 10 μg/mL LPS. A-C) Immunofluorescence assay was used to estimate the expression of Iba-1, CD11b and BDNF in BV2 cells, based on the fluorescence intensity; Iba-1, CD11b or BDNF, red color; DAPI, blue color.

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