Brd4 Antibody - #DF2905

FIGURE 2 | SN-38 inhibited BRD4 (BD1) and BRD4 (BD1) in a reversible manner, SN-38 can bind BRD4 and inhibit cell proliferation in K562 cells.(C) Protein levels of BRD4 in K562 cells treated with SN-38 using CETSA.

FIGURE 3 SN-38 regulated the expression of apoptotic related protein and induced apoptosis of K562 cells. (A) The apoptotic percentage of K562 cells treated with different doses of SN-38; (B), (C) Expression levels of apoptosis-related proteins in K562 cells treated with different doses of SN-38 for 48 h. Data were shown as mean ± SD with three times replication. **p < 0.01 were considered statistically significant compared to control group.

Fig. 4. The role of BRD4 in the development of morphine tolerance. The mRNA level (a) and protein level (b) of BRD4 in spinal cord (****p < 0.0001 vs. NS group. n = 6 per group). (c) Representative photomicrographs of the expression of BRD4 (red) in microglia (IBA-1, green), neurons (NeuN, green) and astrocytes (GFAP, green) of spinal dorsal horn in Naïve, NS and MT group rats (n = 3 rats per group). (d) Co-administration of morphine (10 μg) with JQ-1 attenuated morphine tolerance as evidenced by the measurement of tail-flick latency. (***p < 0.001, ****p < 0.0001 vs. Vehicle group, ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. MT + Vehicle group, $$ p < 0.01 vs. M + 15 μg JQ-1 group. n = 6 per group). Real-time PCR (e) and Western blot (f) analysis showed the mRNA level and protein level of BRD4 in spinal cord after injection of 30 μg JQ-1 (****p < 0.0001 vs. Vehicle group, ####p < 0.0001 vs. MT + Vehicle group. n = 6 per group).

Fig. 5 Effects of TBB on BRD4 phosphorylation in vitro and in vivo. A Timeline of fibroblast treatment. B–D Representative protein expression and quantitative analysis of p-BRD4 and BRD4 protein levels in fibroblasts incubated with TGF-β1 and/or TBB for 72 h, determined by Western blotting (n = 3). *P 

FIGURE 6 Xylitol attenuates renal fibrosis in vivo and in vitro by inhibiting BRD4 protein. (a) Left kidney qPCR experiments in mice of the sham group, sham + Xylitol group, UUO group, and UUO + Xylitol group. (n = 8). (b) Molecular docking to evaluate the interaction between xylitol and BRD4. (c, d) Immunofluorescence experiments against BRD4 in the control group, Xylitol group, TGF-β1 group, TGF-β1 + Xylitol group (IF, scale bar, 100 μm, magnification, ×400, n = 5). (e, f) Immunofluorescence experiments against BRD4 in the sham group, sham + Xylitol group, UUO group, and UUO + Xylitol group of mice left kidneys for BRD4 protein expression in tubular epithelial cells (IF, scale bar, 100 μm, magnification, ×400, n = 5). (g, h) Control group, TGF-β1 group, TGF-β1 + xylitol TGF-β1 + JQ1, and TGF-β1 + JQ1 + Xylitol groups for protein imprinting experiments of representative fibronectin by HK-2 cells (n = 4). All of the in vitro experiments were conducted at least three times. Data are presented as mean ± SEM. *p