BMAL1 Antibody - #DF10308

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产品: BMAL1 抗体
货号: DF10308
描述: Rabbit polyclonal antibody to BMAL1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O00327
RRID: AB_2840886

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
BMAL1 Antibody detects endogenous levels of total BMAL1.
RRID:
AB_2840886
引用格式: Affinity Biosciences Cat# DF10308, RRID:AB_2840886.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARNT like protein 1 brain and muscle; Arntl; Aryl hydrocarbon receptor nuclear translocator like; Aryl hydrocarbon receptor nuclear translocator like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic helix loop helix PAS orphan MOP3; Basic helix loop helix PAS protein MOP3; Basic-helix-loop-helix-PAS protein MOP3; bHLH PAS protein JAP3; bHLH-PAS protein JAP3; bHLHe5; BMAL 1; BMAL1_HUMAN; BMAL1c; Brain and muscle ARNT like 1; Brain and muscle ARNT-like 1; CG8727 PA; Class E basic helix-loop-helix protein 5; cycle; JAP 3; JAP3; Member of PAS protein 3; Member of PAS superfamily 3; MGC47515; MOP 3; MOP3; PAS domain-containing protein 3; PASD 3; PASD3; TIC;

抗原和靶标

免疫原:

A synthesized peptide derived from human BMAL1, corresponding to a region within the internal amino acids.

基因/基因ID:
ARNTL(406)

研究领域

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

文献引用

1). Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice. Cell communication and signaling : CCS, 2024 (PubMed: 38491517) [IF=8.4]
2). Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. Acta pharmacologica Sinica, 2022 (PubMed: 33850278) [IF=6.9]
3). Protective effect of melatonin against metabolic disorders and neuropsychiatric injuries in type 2 diabetes mellitus mice. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38851097) [IF=6.7]
4). Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease. Frontiers in Pharmacology, 2021 (PubMed: 33790796) [IF=5.6]

Application: WB    Species: Mice    Sample: liver tissue and RAW264.7 cells

FIGURE 1 Effects of ethanol on Bmal1 expression in liver tissue and RAW264.7 cells. (A) The expression of Bmal1 in liver tissues was detected by IHC analysis. Each group presented a representative view. (B) The Bmal1 protein and mRNA levels in liver tissues were analyzed by western blot and q RT-PCR. (C) Immunofluorescence double-staining (IF) was used to analyze the representative co-localization of the immune reactivity of Bmal1 and macrophage CD68 in liver tissues. (D) The Bmal1 protein and mRNA levels in ethanol-induced RAW264.7 cells were analyzed by western blot and q RT-PCR. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 vs. CD-fed group or control group.

Application: IF/ICC    Species: Mice    Sample: liver tissue and RAW264.7 cells

FIGURE 1 Effects of ethanol on Bmal1 expression in liver tissue and RAW264.7 cells. (A) The expression of Bmal1 in liver tissues was detected by IHC analysis. Each group presented a representative view. (B) The Bmal1 protein and mRNA levels in liver tissues were analyzed by western blot and q RT-PCR. (C) Immunofluorescence double-staining (IF) was used to analyze the representative co-localization of the immune reactivity of Bmal1 and macrophage CD68 in liver tissues. (D) The Bmal1 protein and mRNA levels in ethanol-induced RAW264.7 cells were analyzed by western blot and q RT-PCR. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 vs. CD-fed group or control group.

Application: IHC    Species: Mice    Sample: liver tissue and RAW264.7 cells

FIGURE 1 Effects of ethanol on Bmal1 expression in liver tissue and RAW264.7 cells. (A) The expression of Bmal1 in liver tissues was detected by IHC analysis. Each group presented a representative view. (B) The Bmal1 protein and mRNA levels in liver tissues were analyzed by western blot and q RT-PCR. (C) Immunofluorescence double-staining (IF) was used to analyze the representative co-localization of the immune reactivity of Bmal1 and macrophage CD68 in liver tissues. (D) The Bmal1 protein and mRNA levels in ethanol-induced RAW264.7 cells were analyzed by western blot and q RT-PCR. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 vs. CD-fed group or control group.
5). IL-17A Induces Circadian Disruptions Through the Epigenetic Repression of BMAL1 in Mice With Alzheimer's Disease. Journal of cellular and molecular medicine, 2025 (PubMed: 40208086) [IF=5.3]
6). Potential Role of Bmal1 in Lipopolysaccharide-Induced Depression-Like Behavior and its Associated "Inflammatory Storm". Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 2024 (PubMed: 38305948) [IF=5.2]
7). Shuangxia Decoction attenuates sleep disruption in 5×FAD mice through neuroinflammation inhibition: An integrative analysis of transcriptomic and molecular biology investigations. Journal of ethnopharmacology, 2025 (PubMed: 40101857) [IF=4.8]
8). Qibai Pingfei Capsule ameliorated inflammation in chronic obstructive pulmonary disease (COPD) via HIF-1 α/glycolysis pathway mediated of BMAL1. International immunopharmacology, 2025 (PubMed: 39579541) [IF=4.8]

Application: WB    Species: Rat    Sample:

Fig. 3. Effects of QBPF on BMAL1 and HIF-1α in COPD rats. (A–B) Molecular docking and co-immunoprecipitation on BMAL1 and HIF-1α. (C-E) WB was performed to assess the expression of BMAL1 and HIF-1α in lung tissues of COPD rats. (F-G) The gene expression levels of BMAL1 and HIF-1α in lung tissues of mice were detected by RT-qPCR. Data were presented as the mean ± SEM. *p < 0.05, **p < 0.01.
9). Role of Bmal1 in Type 2 Diabetes Mellitus-Related Glycolipid Metabolic Disorder and Neuropsychiatric Injury: Involved in the Regulation of Synaptic Plasticity and Circadian Rhythms. Molecular neurobiology, 2023 (PubMed: 37126129) [IF=4.6]
10). Dynamic and functional analyses of exosomal miRNAs regulating cellular microenvironment of ovarian cancer cells. Journal of ovarian research, 2025 (PubMed: 39930447) [IF=4.0]
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