DYR1B Antibody - #AF0607

(1)  
产品: DYR1B 抗体
货号: AF0607
描述: Rabbit polyclonal antibody to DYR1B
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Rabbit, Dog
分子量: 70kDa; 69kD(Calculated).
蛋白号: Q9Y463
RRID: AB_2834359

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
DYR1B Antibody detects endogenous levels of total DYR1B.
RRID:
AB_2834359
引用格式: Affinity Biosciences Cat# AF0607, RRID:AB_2834359.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AOMS3; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B; Dual specificity tyrosine phosphorylation regulated kinase 1B; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; DYR 1B; DYR1A; DYR1B; DYR1B_HUMAN; DYRK 1B; Dyrk1b; Minibrain related kinase; Minibrain-related kinase; MIRK; Mirk protein kinase;

抗原和靶标

免疫原:
Uniprot:

Q9Y463(DYR1B_HUMAN) >>Visit HPA database.

基因/基因ID:
DYRK1B(9149)
表达:
Q9Y463 DYR1B_HUMAN:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

描述:
DYRK1B is a member of the DYRK family of protein kinases. DYRK1B contains a bipartite nuclear localization signal and is found mainly in muscle and testis. The protein is proposed to be involved in the regulation of nuclear functions. Three isoforms of DYRK1B have been identified differing in the presence of two alternatively spliced exons within the catalytic domain.
序列:
MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y463 作为底物

Site PTM Type Enzyme
S49 Phosphorylation
K57 Acetylation
Y63 Phosphorylation
Y64 Phosphorylation
Y111 Phosphorylation
K119 Ubiquitination
K127 Ubiquitination
K256 Ubiquitination
Y271 Phosphorylation Q9Y463 (DYRK1B)
Y273 Phosphorylation Q9Y463 (DYRK1B)
S276 Phosphorylation
S421 Phosphorylation Q9Y463 (DYRK1B) , P28482 (MAPK1)
Y485 Phosphorylation
S486 Phosphorylation
S576 Phosphorylation

翻译修饰 - Q9Y463 作为激酶

Substrate Site Source
O75533 (SF3B1) T434 Uniprot
P20823 (HNF1A) S249 Uniprot
P24385 (CCND1) T286 Uniprot
P24385 (CCND1) T288 Uniprot
P38936 (CDKN1A) S153 Uniprot
P46527 (CDKN1B) S10 Uniprot
Q02363 (ID2) T27 Uniprot
Q99801 (NKX3-1) S185 Uniprot
Q9UQL6 (HDAC5) S279 Uniprot
Q9Y463 (DYRK1B) Y271 Uniprot
Q9Y463 (DYRK1B) Y273 Uniprot
Q9Y463 (DYRK1B) S421 Uniprot

研究背景

功能:

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).

翻译修饰:

Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

亚基结构:

Dimer. Interacts with DCOHM, MAP2K3/MKK3, RANBP9 and TCF1/HNF1A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with DCAF7.

蛋白家族:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.