CCR6 Antibody - #DF10207

Fig. 5. CCL20 orchestrated the invasiveness and metastasis of BC. (A) Representative images of CCL20 in tumor samples harvested from the patients with BC and MMTV-PyMT mice. Scale bars: 100 µm. (B) Correlation analysis of CCL20 and CCR6. (C) Quantification of CCR6 protein expression in the cells transfected with empty vector, CCL20 OE BC cells and CCL20 KO BC cells (n = 3). (D) Western blot analysis of the markers for EMT and invadopodium in the cells transfected with empty vector, CCL20 OE BC cells and CCL20 KO BC cells. (E-L) Quantification of markers for EMT and invadopodium (n = 3). Representative immunofluorescence images for the markers of invadopodium including MMP2 (M) and CTTN (N). Scale bars: 10 µm. (O) Schematic diagram for experimental metastasis model. (P) Representative high-resolution micro-CT images for lung capacity in the vector, CCL20 OE and CCL20 KO groups. (Q) The quantification of lung capacity in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). (R) The quantification of loss of lung capacity in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). (S) H&E staining of the livers. Scale bars: 100 µm (upper) and 200 µm (bottom). (T) Numbers of the metastatic nodules in the livers in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). (U) Representative images and H&E staining of the lungs. Scale bars: 100 µm (upper panel) and 200 µm (bottom panel). (V) Numbers of the metastatic nodules in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). *P