CCR3 Antibody - #DF10205

FIG 2 CCR1 expression decreased in A549 cells infected with RSV, and the CCR1 expression level was affected by the level of intracellular viral protein. (A) The expression of CCL5 receptors in A549 cells infected with RSV was analyzed by Western blotting. (B) A549 cells were transfected with 500 ng of CCR1, CCR3, CCR5, or an empty plasmid. Whole protein was extracted, and the transfection efficiency was verified by Western blotting. (C and D) The viral load of A549 cells overexpressing CCR1, CCR3, or CCR5 was analyzed by fluorescence microscopy (C) and Western blotting (D). (E) Western blot showing the effect of CCR5 gene knockout in A549 cells that had been infected with RSV and incubated for 24 h. (F and G) The number of intracellular viruses was determined by fluorescence microscopy, and the level of intracellular viral proteins was detected by Western blotting (G). Cells were transfected for 30 h and then infected with RSV for 24 h. (Unpaired t test: *, P 

Figure 1. |SIRT1 decreases protein O-GlcNAcylation of tau in cells.(B) shSIRT1 plasmids or its control plasmids were transfected into HEK-293T cells. Recombinant human tau441 was immunoaffinity-purified with anti-GFP antibody from cells. Quantitative analysis of relative O-GlcNAcylation levels of tau after being normalized with total tau is shown as mean ± S.D. (n=3), ***, p < 0.001.

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.