C3AR1 Antibody - #DF10198

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产品: C3AR1 抗体
货号: DF10198
描述: Rabbit polyclonal antibody to C3AR1
应用: WB
反应: Human
预测: Rabbit
分子量: 54 kDa; 54kD(Calculated).
蛋白号: Q16581
RRID: AB_2840777

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Rabbit(83%)
克隆:
Polyclonal
特异性:
C3AR1 Antibody detects endogenous levels of total C3AR1.
RRID:
AB_2840777
引用格式: Affinity Biosciences Cat# DF10198, RRID:AB_2840777.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AZ 3B; AZ3B; C3a anaphylatoxin chemotactic receptor; C3A R; C3A R1; C3a-R; C3AR; C3AR_HUMAN; C3AR1; C3R1; Complement component 3 receptor 1; Complement component 3a receptor 1; HNFAG09;

抗原和靶标

免疫原:
Uniprot:

Q16581(C3AR_HUMAN) >>Visit HPA database.

基因/基因ID:
C3AR1(719)
表达:
Q16581 C3AR_HUMAN:

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

序列:
MASFSAETNSTDLLSQPWNEPPVILSMVILSLTFLLGLPGNGLVLWVAGLKMQRTVNTIWFLHLTLADLLCCLSLPFSLAHLALQGQWPYGRFLCKLIPSIIVLNMFASVFLLTAISLDRCLVVFKPIWCQNHRNVGMACSICGCIWVVAFVMCIPVFVYREIFTTDNHNRCGYKFGLSSSLDYPDFYGDPLENRSLENIVQPPGEMNDRLDPSSFQTNDHPWTVPTVFQPQTFQRPSADSLPRGSARLTSQNLYSNVFKPADVVSPKIPSGFPIEDHETSPLDNSDAFLSTHLKLFPSASSNSFYESELPQGFQDYYNLGQFTDDDQVPTPLVAITITRLVVGFLLPSVIMIACYSFIVFRMQRGRFAKSQSKTFRVAVVVVAVFLVCWTPYHIFGVLSLLTDPETPLGKTLMSWDHVCIALASANSCFNPFLYALLGKDFRKKARQSIQGILEAAFSEELTRSTHCPSNNVISERNSTTV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
83
Bovine
75
Horse
67
Sheep
67
Dog
50
Chicken
44
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16581 作为底物

Site PTM Type Enzyme
S266 O-Glycosylation
S266 Phosphorylation
S349 Phosphorylation
S459 Phosphorylation
T463 Phosphorylation
S465 Phosphorylation
T466 Phosphorylation
S470 Phosphorylation
S475 Phosphorylation
S479 Phosphorylation
T480 Phosphorylation
T481 Phosphorylation

研究背景

功能:

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

翻译修饰:

Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.

O-glycosylated.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

亚基结构:

Interacts with VGF-derived peptide TLQP-21 (By similarity).

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

文献引用

1). Aristolochic acid I promotes the invasion and migration of hepatocellular carcinoma cells by activating the C3a/C3aR complement system. TOXICOLOGY LETTERS (PubMed: 32898628) [IF=3.5]

Application: WB    Species: mouse    Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (A) Enzyme-linked immunosorbent assay was performed to measure AAI-induced excess C3a secretion (B) Western blot assays were performed to measure the expression of the C3aR protein.

Application: IF/ICC    Species: mouse    Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (D) Immunofluorescence images stained for C3aR (green) and DAPI (blue) Scale bar, 50 μm. Each bar represents the mean ±SD based on three replicates. P < 0.05 was considered to be statistically significant. ** compared with vehicle control (0 μM), P <0.01; ***compared with vehicle control (0 μM), P < 0.001; ****compared with vehicle control(0 μM), P < 0.0001

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