SFRP1 Antibody - #DF10172

Fig. 1 The mechanism of regulatory relationships among EZH2, SFRP1, and WNT5A. (A) The miR26a level in C4-2B and C4-2B EnzR cells. n = 3, mean ± SD, ***p 

FIGURE 8 Sohlh1 is negatively correlated with the expression of Nestin and positively correlated with the expression of SFRP1 in glioma tissues. (A) Representative IHC images of Sohlh1, nestin, SFRP1 in glioma samples. (B) Correlation analysis of Sohlh1 and Nestin, SFRP1 in glioma clinical samples. Scale bars indicate 20 μm. (C) A schematic model showing that Sohlh1 directly regulates SFRP1 expression and inhibits Wnt/β-catenin signalling pathway activation, thereby repressing the GSLC stemness and promoting the GSLC differentiation. Statistical results were calculated using a two-tailed Student's t test.

FIGURE 6 Sohlh1 up-regulates the expression of SFRP1 in GSLCs. (A) Expression of SFRP1 in normal tissues (left) and glioma tissues (right) from TCGA database. (B) Correlation between Sohlh1 and SFRP1 expression levels in gliomas based on CGGA database (http://www.cgga.org.cn/) (in mRNASeq−325). q-PCR analysis for mRNA levels of SFRP1 in GSLCs (C) and tumour tissues (F). Western-blot analysis for protein levels of SFRP1 in GSLCs (D, E) and tumour tissues (G, H). (I) Representative IHC images of Sohlh1 and SFRP1 in tumour tissues. Scale bars indicate 20 μm. (J) Potential binding sites of Sohlh1 in SFRP1 promotor were predicted by JASPAR. (K, L) ChIP analysis of forced Sohlh1 expression U87MG GSLCs using anti-Sohlh1 antibody for SFRP1 promoter. (M) Luciferase report assay was performed to detect the effecf of Sohlh1 on transcriptional activity of SFRP1 in U87MG GSLCs. Statistical results were calculated using a two-tailed Student's t test. *p