COLEC12 Antibody - #DF10165

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产品: COLEC12 抗体
货号: DF10165
描述: Rabbit polyclonal antibody to COLEC12
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 82 kDa; 82kD(Calculated).
蛋白号: Q5KU26
RRID: AB_2840744

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
COLEC12 Antibody detects endogenous levels of total COLEC12.
RRID:
AB_2840744
引用格式: Affinity Biosciences Cat# DF10165, RRID:AB_2840744.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CL P1; CL-P1; CLP1; COL12_HUMAN; COLEC 12; colec12; Collectin placenta 1; Collectin placenta protein 1; Collectin sub family member 12; Collectin-12; hCL P1; hCL-P1; NSR2; Nurse cell scavenger receptor 2; SCARA4; Scavenger receptor class A member 4; Scavenger receptor with C type lectin; Scavenger receptor with C-type lectin; SRCL;

抗原和靶标

免疫原:
Uniprot:

Q5KU26(COL12_HUMAN) >>Visit HPA database.

基因/基因ID:
COLEC12(81035)
表达:
Q5KU26 COL12_HUMAN:

Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed in placenta. Moderately expressed in heart, skeletal muscle, small intestine and lung. Weakly expressed in brain, colon, thymus and kidney. Expressed in nurse-like cells. Expressed in reactive astrocytes and vascular/perivascular cells in the brain of Alzheimer patient.

序列:
MKDDFAEEEEVQSFGYKRFGIQEGTQCTKCKNNWALKFSIILLYILCALLTITVAILGYKVVEKMDNVTGGMETSRQTYDDKLTAVESDLKKLGDQTGKKAISTNSELSTFRSDILDLRQQLREITEKTSKNKDTLEKLQASGDALVDRQSQLKETLENNSFLITTVNKTLQAYNGYVTNLQQDTSVLQGNLQNQMYSHNVVIMNLNNLNLTQVQQRNLITNLQRSVDDTSQAIQRIKNDFQNLQQVFLQAKKDTDWLKEKVQSLQTLAANNSALAKANNDTLEDMNSQLNSFTGQMENITTISQANEQNLKDLQDLHKDAENRTAIKFNQLEERFQLFETDIVNIISNISYTAHHLRTLTSNLNEVRTTCTDTLTKHTDDLTSLNNTLANIRLDSVSLRMQQDLMRSRLDTEVANLSVIMEEMKLVDSKHGQLIKNFTILQGPPGPRGPRGDRGSQGPPGPTGNKGQKGEKGEPGPPGPAGERGPIGPAGPPGERGGKGSKGSQGPKGSRGSPGKPGPQGSSGDPGPPGPPGKEGLPGPQGPPGFQGLQGTVGEPGVPGPRGLPGLPGVPGMPGPKGPPGPPGPSGAVVPLALQNEPTPAPEDNGCPPHWKNFTDKCYYFSVEKEIFEDAKLFCEDKSSHLVFINTREEQQWIKKQMVGRESHWIGLTDSERENEWKWLDGTSPDYKNWKAGQPDNWGHGHGPGEDCAGLIYAGQWNDFQCEDVNNFICEKDRETVLSSAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q5KU26 作为底物

Site PTM Type Enzyme
Y16 Phosphorylation
T69 Phosphorylation
T74 Phosphorylation
T78 Phosphorylation
T104 Phosphorylation
S106 Phosphorylation
S109 Phosphorylation
T110 Phosphorylation
K128 Acetylation
K131 Acetylation
R149 Methylation
S398 Phosphorylation
K425 Acetylation
Y687 Phosphorylation

研究背景

功能:

Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid-beta in Alzheimer disease.

细胞定位:

Membrane>Single-pass type II membrane protein.
Note: Forms clusters on the cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed in placenta. Moderately expressed in heart, skeletal muscle, small intestine and lung. Weakly expressed in brain, colon, thymus and kidney. Expressed in nurse-like cells. Expressed in reactive astrocytes and vascular/perivascular cells in the brain of Alzheimer patient.

亚基结构:

The extracellular domain forms a stable trimer. The extracellular domain interacts with fibrillar amyloid-beta peptide.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

文献引用

1). Integrated bioinformatics analyses of key genes involved in hepatocellular carcinoma immunosuppression. Oncology Letters (PubMed: 34691257) [IF=2.9]

Application: WB    Species: Human    Sample: liver tissues and HCC tissues

Figure 4. Validation of COLEC12, MMP12 and MUC12 mRNA and protein levels. (A) Comparison of COLEC12 mRNA levels between HCC tissues (n=17) and cirrhosis (n=20) or normal liver tissues (n=6) in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of COLEC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (B) The comparisons of the MMP12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148). (C) The comparisons of the MUC12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of MUC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (D) The comparisons of the mRNA and protein expression levels of COLEC12, MUC12 and MMP12 between tumor samples (HCC tissues, n=4) and normal (corresponding adjacent normal liver tissues, n=4) samples of HCC patients from the Second Affiliated Hospital of Nanchang University. *P<0.05 and **P<0.01 vs. normal or cirrhosis, respectively. COLEC12, collectin-12 gene, MMP12, matrix metalloproteinase-12 gene; MUC12, mucin-12 gene; HCC, hepatocellular carcinoma; GEO, Gene Expression Omnibus.

Application: WB    Species: Human    Sample: HCC tissues and normal liver tissues

Figure 4. Validation of COLEC12, MMP12 and MUC12 mRNA and protein levels. (A) Comparison of COLEC12 mRNA levels between HCC tissues (n=17) and cirrhosis (n=20) or normal liver tissues (n=6) in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of COLEC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (B) The comparisons of the MMP12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148). (C) The comparisons of the MUC12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of MUC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (D) The comparisons of the mRNA and protein expression levels of COLEC12, MUC12 and MMP12 between tumor samples (HCC tissues, n=4) and normal (corresponding adjacent normal liver tissues, n=4) samples of HCC patients from the Second Affiliated Hospital of Nanchang University. *P<0.05 and **P<0.01 vs. normal or cirrhosis, respectively. COLEC12, collectin-12 gene, MMP12, matrix metalloproteinase-12 gene; MUC12, mucin-12 gene; HCC, hepatocellular carcinoma; GEO, Gene Expression Omnibus.

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