MOBL3 Antibody - #AF0726

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产品: MOBL3 抗体
货号: AF0726
描述: Rabbit polyclonal antibody to MOBL3
应用: WB
反应: Human, Mouse, Rat
预测: Zebrafish, Chicken, Xenopus
分子量: 26kDa; 26kD(Calculated).
蛋白号: Q9Y3A3
RRID: AB_2834354

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(88%), Chicken(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
MOBL3 Antibody detects endogenous levels of total MOBL3.
RRID:
AB_2834354
引用格式: Affinity Biosciences Cat# AF0726, RRID:AB_2834354.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2C4D; CGI 95; class II mMOB1; MGC12264; MOB family member 4, phocein; MOB like protein phocein; MOB1; mob1 homolog 3; MOB1, Mps One Binder kinase activator like 3; Mob3; MOB4; MOBKL3; MOBL3_HUMAN; mps one binder kinase activator like 3; Mps one binder kinase activator-like 3; Phocein Mob like protein; PHOCN; PREI3; Preimplantation protein 3; RCJMB04_1o21;

抗原和靶标

免疫原:
Uniprot:

Q9Y3A3(PHOCN_HUMAN) >>Visit HPA database.

基因/基因ID:
MOB4(25843)
描述:
This gene was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
序列:
MVMAEGTAVLRRNRPGTKAQDFYNWPDESFDEMDSTLAVQQYIQQNIRADCSNIDKILEPPEGQDEGVWKYEHLRQFCLELNGLAVKLQSECHPDTCTQMTATEQWIFLCAAHKTPKECPAIDYTRHTLDGAACLLNSNKYFPSRVSIKESSVAKLGSVCRRIYRIFSHAYFHHRQIFDEYENETFLCHRFTKFVMKYNLMSKDNLIVPILEEEVQNSVSGESEA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Chicken
100
Zebrafish
88
Xenopus
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y3A3 作为底物

Site PTM Type Enzyme
C134 S-Nitrosylation
K140 Acetylation
K140 Ubiquitination
Y141 Phosphorylation
S147 Phosphorylation
K149 Ubiquitination
K155 Acetylation
T192 Phosphorylation
Y198 Phosphorylation

研究背景

功能:

May play a role in membrane trafficking, specifically in membrane budding reactions.

翻译修饰:

Phosphorylated on serine residues.

细胞定位:

Cytoplasm>Perinuclear region. Membrane>Peripheral membrane protein. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein.
Note: In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds STRN4 (By similarity). Interacts with DNM1 and EPS15 (By similarity). Interacts with nucleoside diphosphate kinase (By similarity). Binds STRN and STRN3. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2. Interacts with CTTNBP2 (By similarity). Interacts with CTTNBP2NL.

蛋白家族:

Belongs to the MOB1/phocein family.

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