(4)
(7)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10099, RRID:AB_2840679.
展开/折叠
CYBA; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit;
抗原和靶标
- P13498 CY24A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGQIEWAMWANEQALASGLILITGGIVATAGRFTQWYFGAYSIVAGVFVCLLEYPRGKRKKGSTMERWGQKYMTAVVKLFGPFTRNYYVRAVLHLLLSVPAGFLLATILGTACLAIASGIYLLAAVRGEQWTPIEPKPRERPQIGGTIKQPPSNPPPRPPAEARKKPSEEEAAVAAGGPPGGPQVNPIPVTDEVV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P13498 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K60 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| Y72 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| Y87 | Phosphorylation | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| T147 | Phosphorylation | P17252 (PRKCA) , Q05655 (PRKCD) | Uniprot |
| K149 | Ubiquitination | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot |
研究背景
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.
Ubiquitinated at Lys-149 likely by RNF145.
Cell membrane.
Note: As unassembled monomer may localize to the endoplasmic reticulum.
Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9).
Belongs to the p22phox family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
文献引用
Application: WB Species: mouse Sample: renal cortex
Application: WB Species: rat Sample: testis
Application: WB Species: Mouse Sample:
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