ZNF148 Antibody - #AF0792

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产品: ZNF148 抗体
货号: AF0792
描述: Rabbit polyclonal antibody to ZNF148
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
蛋白号: Q9UQR1
RRID: AB_2834348

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ZNF148 Antibody detects endogenous levels of total ZNF148.
RRID:
AB_2834348
引用格式: Affinity Biosciences Cat# AF0792, RRID:AB_2834348.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148;

抗原和靶标

免疫原:

A synthesized peptide derived from human ZNF148, corresponding to a region within N-terminal amino acids.

基因/基因ID:
ZNF148(7707)
描述:
ZNF148 a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

文献引用

1). Hsa_circRNA_0101125 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma via miR-143-3p/ZNF148 Axis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2025 (PubMed: 41020579) [IF=4.4]

Application: WB    Species: human    Sample: KYSE150 and ECA109 cell

FIGURE 2. Effect of hsa_circRNA_0101125 knockdown on proliferation, migration, and epithelial marker expression in ESCC cells. (A) RT‐qPCR analysis showing the reduction of hsa_circRNA_0101125 expression in KYSE150 and ECA109 cells after transfection with sh‐hsa_circRNA_0101125. (B) RT‐qPCR analysis of miR‐143‐3p expression following hsa_circRNA_0101125 knockdown. (C) Representative images of scratch wound healing assay in ECA109 cells at 0 h and 24 h. (D‐E) Quantitative analysis of wound closure. (F) Cell viability of ECA109 cells measured by CCK‐8 assay at 24 h and 48 h after knockdown. (G) Western blot analysis of E‐cadherin protein expression in KYSE150 and ECA109 cells after knockdown. Relative expression values represent the gray intensity of E‐cadherin normalized to β‐Actin, based on densitometry analysis using ImageJ software. *p 

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