产品: MYCBP 抗体
货号: DF10076
描述: Rabbit polyclonal antibody to MYCBP
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse
预测: Bovine, Rabbit, Dog, Chicken, Xenopus
分子量: 12 kDa; 12kD(Calculated).
蛋白号: Q99417
RRID: AB_2840656

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Bovine(100%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
MYCBP Antibody detects endogenous levels of total MYCBP.
RRID:
AB_2840656
引用格式: Affinity Biosciences Cat# DF10076, RRID:AB_2840656.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AMY 1; AMY-1; AMY1; Associate of Myc 1; C myc binding protein; C-Myc-binding protein; Cmyc binding protein; MYCBP; MYCBP_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q99417 MYCBP_HUMAN:

Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

序列:
MAHYKAADSKREQFRRYLEKSGVLDTLTKVLVALYEEPEKPNSALDFLKHHLGAATPENPEIELLRLELAEMKEKYEAIVEENKKLKAKLAQYEPPQEEKRAE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Xenopus
91
Chicken
91
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.

细胞定位:

Cytoplasm. Nucleus. Mitochondrion.
Note: Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

亚基结构:

Binds via its C-terminal region to the N-terminal region of MYC. Associates with AKAP1/S-AKAP84. Interacts with MYCBPAP.

蛋白家族:

Belongs to the AMY1 family.

文献引用

1). Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. PLOS Neglected Tropical Diseases, 2017 (PubMed: 36206314) [IF=3.4]

Application: WB    Species: Mouse    Sample:

Fig 1. The dynamic changes of mouse proteins in serum-derived exosomes in response to E. multilocularis infection. (A) and (B) Transmission electron microscopy of the enriched extracellular vesicles (EVs) isolated from the sera of E. multilocularis-infected (A) and uninfected mice (B). (C) The diameter distribution of the purified EVs. (D) Western blotting analysis of mammalian EV-marker CD63 in the purified EVs. (E) Heatmap of differentially abundance proteins between the serum exosomes from E. multilocularis-infected and uninfected mice. (F) Western blotting analysis the abundance of TGF-β1, GPATC8, LRP1, and AMY1 during E. multilocularis infection. https://doi.org/10.1371/journal.pntd.0010814.g001

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