ATOH8 Antibody - #DF10047

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(2)   (3)  
产品: ATOH8 抗体
货号: DF10047
描述: Rabbit polyclonal antibody to ATOH8
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q96SQ7
RRID: AB_2840627

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC : 1:50-200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
ATOH8 Antibody detects endogenous levels of total ATOH8.
RRID:
AB_2840627
引用格式: Affinity Biosciences Cat# DF10047, RRID:AB_2840627.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4933425C05Rik; ATOH8; ATOH8_HUMAN; Atonal homolog 6; atonal homolog 8 (Drosophila); Atonal homolog 8; Basic helix loop helix transcription factor 6; bHLHa21; Class A basic helix-loop-helix protein 21; FLJ14708; FLJ38730; hATH6; Helix loop helix protein mATH-6; Helix-loop-helix protein hATH-6; Math6; Okadin; Protein atonal homolog 8; RGD1561512;

抗原和靶标

免疫原:

A synthesized peptide derived from human ATOH8, corresponding to a region within C-terminal amino acids.

基因/基因ID:
ATOH8(84913)

文献引用

1). Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2020 (PubMed: 32000836) [IF=11.3]

Application: IF/ICC    Species: Human    Sample: SW480 cells

Fig. 1 ATOH8 is a shear stress responsive molecule in mimic circulating colorectal cancer cells. a, b Left, representative immunofluorescence images of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (a) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (b) laminar shear stress (LSS). Right, quantification of single tumour cell fluorescence intensity in various treatment groups was presented. c-f Western blotting (WB) and quantitative polymerase chain reaction (qPCR) analysis of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (c, d) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (e, f) LSS. g Immunofluorescence representation images of different subtypes of CTCs in colorectal cancer patients. Red represents epithelial CTCs, green represents mesenchymal CTC, red and green represent mixed CTC, and purple shade represents different expression levels of ATOH8 in CTCs. h In colorectal cancer patients with hypertension, the number of ATOH8 (+) CTCs (CTCs with ATOH8 expression, including ATOH8 low, moderate and high expression) were higher. i Representative immunohistochemistry images of ATOH8, HK2, GLUT1 and LDHA expression in serial sections of mouse subcutaneous tumour tissue and colon cancer lung metastases. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

Application: WB    Species: Human    Sample: SW480 cells

Fig. 1 ATOH8 is a shear stress responsive molecule in mimic circulating colorectal cancer cells. a, b Left, representative immunofluorescence images of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (a) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (b) laminar shear stress (LSS). Right, quantification of single tumour cell fluorescence intensity in various treatment groups was presented. c-f Western blotting (WB) and quantitative polymerase chain reaction (qPCR) analysis of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (c, d) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (e, f) LSS. g Immunofluorescence representation images of different subtypes of CTCs in colorectal cancer patients. Red represents epithelial CTCs, green represents mesenchymal CTC, red and green represent mixed CTC, and purple shade represents different expression levels of ATOH8 in CTCs. h In colorectal cancer patients with hypertension, the number of ATOH8 (+) CTCs (CTCs with ATOH8 expression, including ATOH8 low, moderate and high expression) were higher. i Representative immunohistochemistry images of ATOH8, HK2, GLUT1 and LDHA expression in serial sections of mouse subcutaneous tumour tissue and colon cancer lung metastases. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001
2). ATOH8 Expression Is Regulated by BMP2 and Plays a Key Role in Human Endometrial Stromal Cell Decidualization. Endocrinology, 2024 (PubMed: 38060684) [IF=3.8]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.