DUS2L Antibody - #AF0430

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产品: DUS2L 抗体
货号: AF0430
描述: Rabbit polyclonal antibody to DUS2L
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 55kDa; 55kD(Calculated).
蛋白号: Q9NX74
RRID: AB_2834339

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%)
克隆:
Polyclonal
特异性:
DUS2L Antibody detects endogenous levels of total DUS2L.
RRID:
AB_2834339
引用格式: Affinity Biosciences Cat# AF0430, RRID:AB_2834339.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2310016K04Rik; dihydrouridine synthase 2 like, SMM1 homolog (S. cerevisiae); DUS2; FLJ20399; SMM1; tRNA dihydrouridine synthase 2 like; Up regulated in lung cancer; URLC8;

抗原和靶标

免疫原:
Uniprot:

Q9NX74(DUS2L_HUMAN) >>Visit HPA database.

基因/基因ID:
DUS2(54920)
表达:
Q9NX74 DUS2L_HUMAN:

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).

描述:
DUS2L Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus2 subfamily.
序列:
MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Pig
91
Bovine
91
Sheep
91
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NX74 作为底物

Site PTM Type Enzyme
S5 Phosphorylation
Y10 Phosphorylation
T24 Phosphorylation
K118 Ubiquitination
S121 Phosphorylation
K123 Ubiquitination
K146 Ubiquitination
K155 Ubiquitination
K170 Ubiquitination
T175 Phosphorylation
T234 Phosphorylation
K266 Ubiquitination
Y274 Phosphorylation
Y283 Phosphorylation
K337 Ubiquitination
K417 Ubiquitination
S445 Phosphorylation
S447 Phosphorylation
K477 Ubiquitination
S484 Phosphorylation
S488 Phosphorylation

研究背景

功能:

Dihydrouridine synthase. Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.

细胞定位:

Cytoplasm. Endoplasmic reticulum.
Note: Mainly at the endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).

亚基结构:

Interacts with EPRS1. Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).

蛋白家族:

Efficient dihydrouridine synthesis requires the presence of both the catalytic domain and the C-terminal RNA-binding DRBM domain.

Belongs to the Dus family. Dus2 subfamily.

限制条款

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