产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3329, RRID:AB_2834333.
展开/折叠
Calcium dependent phospholipid binding protein; CPLA 2; cPLA2 alpha; cPLA2; Cytosolic phospholipase A2; Cytosolic phospholipase A2 group IVA; Lysophospholipase; MGC126350; PA24A_HUMAN; Phosphatidylcholine 2 acylhydrolase; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group 4 A; Phospholipase A2 group IVA (cytosolic calcium dependent); Phospholipase A2 group IVA; PhospholipaseA2; PLA2G4; pla2g4a;
抗原和靶标
Expressed in various tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815).
- P47712 PA24A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P47712 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Phosphorylation | Uniprot | |
Y7 | Phosphorylation | Uniprot | |
T21 | Phosphorylation | Uniprot | |
T31 | Phosphorylation | Uniprot | |
K32 | Ubiquitination | Uniprot | |
C151 | S-Nitrosylation | Uniprot | |
K155 | Ubiquitination | Uniprot | |
T156 | Phosphorylation | Uniprot | |
K171 | Acetylation | Uniprot | |
K176 | Acetylation | Uniprot | |
K176 | Ubiquitination | Uniprot | |
S178 | Phosphorylation | Uniprot | |
S183 | Phosphorylation | Uniprot | |
S228 | Phosphorylation | Uniprot | |
K257 | Ubiquitination | Uniprot | |
T268 | Phosphorylation | Uniprot | |
K271 | Ubiquitination | Uniprot | |
K273 | Ubiquitination | Uniprot | |
K281 | Ubiquitination | Uniprot | |
K283 | Ubiquitination | Uniprot | |
K316 | Ubiquitination | Uniprot | |
Y359 | Phosphorylation | Uniprot | |
S370 | Phosphorylation | Uniprot | |
K371 | Ubiquitination | Uniprot | |
S431 | Phosphorylation | Uniprot | |
S434 | Phosphorylation | Uniprot | |
S435 | Phosphorylation | Uniprot | |
S437 | Phosphorylation | Uniprot | |
S441 | Phosphorylation | Uniprot | |
T447 | Phosphorylation | Uniprot | |
S454 | Phosphorylation | Uniprot | |
Y456 | Phosphorylation | Uniprot | |
S458 | Phosphorylation | Uniprot | |
S463 | Phosphorylation | Uniprot | |
S505 | Phosphorylation | Q16539 (MAPK14) , P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
S508 | Phosphorylation | Uniprot | |
T512 | Phosphorylation | Uniprot | |
S515 | Phosphorylation | Uniprot | |
Y535 | Phosphorylation | Uniprot | |
K541 | Sumoylation | Uniprot | |
K541 | Ubiquitination | Uniprot | |
K595 | Ubiquitination | Uniprot | |
K606 | Ubiquitination | Uniprot | |
K618 | Ubiquitination | Uniprot | |
K691 | Ubiquitination | Uniprot | |
S724 | Phosphorylation | Uniprot | |
S727 | Phosphorylation | O75582 (RPS6KA5) , Q8IW41 (MAPKAPK5) , Q9BUB5 (MKNK1) | Uniprot |
S729 | Phosphorylation | Uniprot | |
S731 | Phosphorylation | Uniprot | |
K741 | Ubiquitination | Uniprot |
研究背景
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Activated by phosphorylation at both Ser-505 and Ser-727.
Cytoplasm. Cytoplasmic vesicle.
Note: Translocates to membrane vesicles in a calcium-dependent fashion.
Expressed in various tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level).
Interacts with KAT5.
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Lipid metabolism > Ether lipid metabolism.
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Lipid metabolism > Linoleic acid metabolism.
· Metabolism > Lipid metabolism > alpha-Linolenic acid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
文献引用
Application: WB Species: Human Sample: platelets
Application: WB Species: Rat Sample: hypoxic beating rat atria
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