Phospho-DRP1 (Ser616) Antibody - #AF8470

FIGURE 6 GSK-3β was found as a key regulatory target of Aβ-induced excessive mitochondrial fission and bone loss. (a) Predicted phosphorylated kinase of DRP1 by iGPS1.0 software. (b) The binding mode of the complex GSK3β with Drp1. The backbone of protein was rendered in tube and colored in red (GSK3β) and cyan (Drp1). GSK3β and Drp1 protein is rendered by the surface. The detail binding mode of GSK3B with Drp1. (c) Immunofluorescence of GSK-3β; p-Drp1S616; DAPI. Scale bars: 5 μm. (d) The Mito Tracker staining to assess the role of GSK-3β inhibitor, LiCl, rescues the length of mitochondria affected by Aβ. Scale bars: 5 μm. (e) TUNEL staining shows the apoptosis of ECs and the quantitative analysis. Scale bar: 50 μm. (f) TUNEL staining to assess the role of LiCl in the apoptosis of ECs in vivo. Scale bars: 50 μm. (g) The assessment of bone mass by μCT and HE staining and quantitative parameters. Scale bar: 200, 50 μm. BMD, Bone mineral density, BV/TV, Bone Volume per Trabecular Volume. Values are presented as mean ± SEM. One-way ANOVA with Bonferroni's multiple comparisons test was performed in d, e, f, g. (****p 

Fig. 6 Mitochondrial regulatory proteins associated with OSCC progression and inhibitory effect of ALT against OSCC cells. A TCGA database evaluated expression patterns across diverse head and neck squamous carcinoma and normal tissues. B Cancer protein target interaction network. C Protein interaction relationship histogram. D ATP synthesis detected in the indicated groups. E Images of immunohistochemistry staining for mitochondrial regulating proteins in adjacent non-cancer tissues and OSCC tissues. Scale bar = 50 µm. F Prognostic graphs illustrating the impact of Drp1 on overall survival in TCGA. G, H Western blot band of Drp1 protein expression after different treatments. I, J p-Drp1 level expressed relative to Drp1 level. (****p 

Fig. 6 Mitochondrial regulatory proteins associated with OSCC progression and inhibitory effect of ALT against OSCC cells. A TCGA database evaluated expression patterns across diverse head and neck squamous carcinoma and normal tissues. B Cancer protein target interaction network. C Protein interaction relationship histogram. D ATP synthesis detected in the indicated groups. E Images of immunohistochemistry staining for mitochondrial regulating proteins in adjacent non-cancer tissues and OSCC tissues. Scale bar = 50 µm. F Prognostic graphs illustrating the impact of Drp1 on overall survival in TCGA. G, H Western blot band of Drp1 protein expression after different treatments. I, J p-Drp1 level expressed relative to Drp1 level. (****p