| 产品: | 磷酸化 14-3-3 sigma (Ser186) 抗体 |
| 货号: | AF8458 |
| 描述: | Rabbit polyclonal antibody to Phospho-14-3-3 sigma (Ser186) |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 28kDa; 28kD(Calculated). |
| 蛋白号: | P31947 |
| RRID: | AB_2840512 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8458, RRID:AB_2840512.
展开/折叠
14 3 3 protein sigma; 14-3-3 protein sigma; 1433S_HUMAN; Epithelial cell marker protein 1; Er; HME 1; HME1; MGC143283; Mkrn3; Mme1; OTTHUMP00000004242; RP23 137L22.11; SFN; SFN protein; Stratifin; YWHAS;
抗原和靶标
Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
- P31947 1433S_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31947 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| C38 | S-Nitrosylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K49 | Acetylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| Y127 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| K159 | Acetylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | P53779 (MAPK10) , P45983 (MAPK8) , P45984 (MAPK9) | Uniprot |
| T207 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot |
研究背景
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression.
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Cytoplasm. Nucleus. Secreted.
Note: May be secreted by a non-classical secretory pathway.
Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Homodimer. Interacts with KRT17 and SAMSN1 (By similarity). Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29 and VPS35. Interacts with GAB2. Interacts with SRPK2. Interacts with COPS6. Interacts with COP1; this interaction leads to proteasomal degradation. Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with PI4KB. Interacts with SLITRK1. Interacts with LRRK2; this interaction is dependent on LRRK2 phosphorylation.
Belongs to the 14-3-3 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
限制条款
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