Phospho-FGFR3 (Tyr724) Antibody - #AF8439

Fig. 3 METTL3 regulates FGFR3 mRNA m6A modification and inhibits FGFR3 mRNA stability. A The METTL3 protein level and m6A level of RNA were detected by western blotting or dot blot in METTL3-knockdown OSCC cells (SCC9 and SCC25). B Representative m6A modification of FGFR3 in OSCC by MeRIP-seq (data from our previous study).C MeRIP-qPCR showed that relative FGFR3 m6A level was significantly decreased after METTL3 knockdown in SCC9 and SCC25 cells. D The FGFR3 protein and mRNA levels, and p-FGFR3 protein levels were significantly increased in METTL3 knockdown OSCC cells (SCC9 and SCC25). E FGFR3 mRNA stability was significantly decreased in METTL3-knockdown OSCC cells after treated with actinomycin D. F. FGFR protein stability was no significant changes between control cells and METTL3-knockdown OSCC cells by cycloheximide assay. Quantification of the protein optical density by ImageJ.

Fig. 3. Effects of LRG on the gene expression in PA-treated LPS-induced RAW264.7 cells. (A) Effects of LRG on secretion of FGF21 in culture supernatant; (B) Effects of LRG on mRNA expression of FGF21; (C) Effects of LRG on mRNA expression of ACC1; (D) Effects of LRG on the protein expression of FGF21, p-FGFR2 and p-FGFR3; (E) Quantification of Fig. 3D; (F) Effect of LRG on protein expression of AMPK pathway; (G) Quantification of Fig. 3F. #p < 0.05, ##p < 0.01 vs CON; $p < 0.05, $$p < 0.01 vs LPS þ HGHP; &p < 0.05, &&p < 0.01 vs PA þ LPS þ LRG (L); *p < 0.05, **p < 0.01 vs PA þ LPS þ LRG (M).