Phospho-SYK (Tyr525+Tyr526) Antibody - #AF8404

Fig. 3. SAP130 derived from TEC ferroptosis promoted macrophage M1 polarization by activating the Mincle/Syk/NF-kB pathway. A. Representative immunofluorescence images of BMDM co-cultured with PBS/LPS pre-stimulated PRTCs with staining for nuclei(blue), iNOS(red) and CD206(green); B. The gene mRNA level of CD80, HLA, TGF-β and CD206 of different time points in THP-1 co-cultured with PBS/LPS pre-stimulated HK-2; C. The protein level of CD163, Arg, Mincle and iNOS in BMDMs; D. Representative immunofluorescence images of Mincle in BMDMs co-cultured with PBS, erastin, LPS and LPS + Fer-1 pre-stimulated PRTCs; E. The gene mRNA level of Mincle in different groups; F. The protein level of Mincle, iNOS, Syk, p-Syk, p65, and p-p65 in BMDMs; G. The gene mRNA level of TNF-α, IL-1 and IL-6 in THP-1 co-cultured with PBS/LPS pre-stimulated HK-2 (n = 3 per group). Data are presented as means ± SEM per group. *P < 0.05, **P < 0.01.

Figure 5. Inhibitory effects of HXZQ-OL on the phosphorylation of FceRI-induced degranulation signalling cascades (A, B, C) and MAPKs (D) in IgE/Ag-mediated RBL- 2H3 cells. IgE-sensitized RBL-2H3 cells were incubated with HXZQ-OL for 30min, followed by DNP-BSA challenge for 10min. The expression levels of p-Fyn, p-Lyn, p- Syk, p-PLCc1, p-PLCc2, p-PKCd, p-Gabs, p-PI3K, p-Akt, p-ERK1/2, p-p38 and p-JNK1/2 were normalized to total proteins, respectively. The data were expressed as the mean±SD values of three independent experiments. ? p<0.05 and ?? p<0.01.

Figure 1 . Overexpression of FGF21 inhibits SYK phosphorylation and NLRP3 inflammasome activation in the aortas of diabetic mice and HG-induced VSMCs (A,B) FGF21 levels in serum and aortic tissue homogenates detected by ELISA. (C‒F) Representative immunofluorescence images (400×, scale bar: 20 μm) and summarized integrated density of NLRP3 and ASC, NLRP3 and caspase-1 colocalization in aortas. (G‒J) Representative western blot images and summarized data showing the phosphorylation of SYK, the production of NLRP3 dimers, and the expression of NLRP3 in aortas. (K) The summarized data showing the activity of caspase-1 in aortas. (L‒O) Representative western blot images and summarized data showing the phosphorylation of SYK, the production of the NLRP3 dimer, and the expression of NLRP3 in VSMCs. (P) The summarized data showing the activity of caspase-1 in VSMCs. (Q‒T) Representative immunofluorescence images and summarized integrated density of NLRP3 and ASC, NLRP3 and caspase-1 colocalization (800×, scale bar: 10 μm) in VSMCs. n=6 in mice; n=3 in VSMCs. *P