产品: | 磷酸化 c Abl (Tyr245)[Tyr226] 抗体 |
货号: | AF3037 |
描述: | Rabbit polyclonal antibody to Phospho-c Abl (Tyr245)[Tyr226] |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat, Monkey |
预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
分子量: | 135kDa; 123kD(Calculated). |
蛋白号: | P00519 |
RRID: | AB_2834326 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3037, RRID:AB_2834326.
展开/折叠
Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine protein kinase 1; Abl 1; ABL; ABL proto oncogene 1 non receptor tyrosine kinase; ABL1; ABL1_HUMAN; bcr/abl; bcr/c abl oncogene protein; c ABL; c abl oncogene 1 non receptor tyrosine kinase; c abl oncogene 1 receptor tyrosine kinase; c ABL1; JTK7; p150; Proto oncogene tyrosine protein kinase ABL1; Proto-oncogene c-Abl; Tyrosine-protein kinase ABL1; v abl Abelson murine leukemia viral oncogene homolog 1; v abl;
抗原和靶标
- P00519 ABL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00519 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S43 | Phosphorylation | Uniprot | |
S50 | Phosphorylation | Uniprot | |
Y70 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P06241 (FYN) , P00519 (ABL1) | Uniprot |
S75 | Phosphorylation | Uniprot | |
Y93 | Phosphorylation | Uniprot | |
S113 | Phosphorylation | Uniprot | |
Y115 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P06241 (FYN) | Uniprot |
T117 | Phosphorylation | Uniprot | |
Y128 | Phosphorylation | P06241 (FYN) , P08631 (HCK) , P07948 (LYN) | Uniprot |
Y139 | Phosphorylation | P07948 (LYN) , P08631 (HCK) , P06241 (FYN) | Uniprot |
S142 | Phosphorylation | Uniprot | |
Y167 | Phosphorylation | Uniprot | |
Y172 | Phosphorylation | P07948 (LYN) , P08631 (HCK) , P06241 (FYN) | Uniprot |
Y174 | Phosphorylation | Uniprot | |
S180 | Phosphorylation | Uniprot | |
Y185 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P06241 (FYN) | Uniprot |
S187 | Phosphorylation | Uniprot | |
Y215 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P06241 (FYN) | Uniprot |
T224 | Phosphorylation | Uniprot | |
Y226 | Phosphorylation | P06241 (FYN) , P08631 (HCK) , P12931 (SRC) , P07948 (LYN) , P00519 (ABL1) | Uniprot |
S229 | Phosphorylation | Uniprot | |
Y232 | Phosphorylation | Uniprot | |
T243 | Phosphorylation | Uniprot | |
Y253 | Phosphorylation | Uniprot | |
Y257 | Phosphorylation | Uniprot | |
Y264 | Phosphorylation | Uniprot | |
S265 | Phosphorylation | Uniprot | |
T267 | Phosphorylation | Uniprot | |
K274 | Acetylation | Uniprot | |
K285 | Ubiquitination | Uniprot | |
T306 | Phosphorylation | Uniprot | |
Y312 | Phosphorylation | Uniprot | |
T315 | Phosphorylation | Uniprot | |
Y320 | Phosphorylation | Uniprot | |
T389 | Phosphorylation | Uniprot | |
T392 | Phosphorylation | Uniprot | |
Y393 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , A0A173G4P4 (Abl fusion) , P06241 (FYN) , P12931 (SRC) , P00519 (ABL1) | Uniprot |
T394 | Phosphorylation | Uniprot | |
K400 | Ubiquitination | Uniprot | |
Y413 | Phosphorylation | Uniprot | |
S417 | Phosphorylation | Uniprot | |
S446 | Phosphorylation | Q13315 (ATM) | Uniprot |
S456 | Phosphorylation | Uniprot | |
Y456 | Phosphorylation | Uniprot | |
Y469 | Phosphorylation | Uniprot | |
K512 | Ubiquitination | Uniprot | |
T532 | Phosphorylation | Uniprot | |
S535 | Phosphorylation | Uniprot | |
S559 | Phosphorylation | Uniprot | |
S569 | Phosphorylation | Uniprot | |
T597 | Phosphorylation | Uniprot | |
S601 | Phosphorylation | Uniprot | |
T610 | Phosphorylation | Uniprot | |
T613 | Phosphorylation | Uniprot | |
S618 | Phosphorylation | Q13177 (PAK2) | Uniprot |
S619 | Phosphorylation | Q13177 (PAK2) | Uniprot |
S620 | Phosphorylation | Uniprot | |
S642 | Phosphorylation | Uniprot | |
T649 | Phosphorylation | Uniprot | |
T653 | Phosphorylation | Uniprot | |
S659 | Phosphorylation | Uniprot | |
S676 | Phosphorylation | Uniprot | |
S679 | Phosphorylation | Uniprot | |
S683 | Phosphorylation | Uniprot | |
K689 | Methylation | Uniprot | |
S690 | Phosphorylation | Uniprot | |
T692 | Phosphorylation | Uniprot | |
S696 | Phosphorylation | Uniprot | |
T700 | Phosphorylation | Uniprot | |
S708 | Phosphorylation | Uniprot | |
S709 | Phosphorylation | Uniprot | |
S710 | Phosphorylation | Uniprot | |
K711 | Acetylation | Uniprot | |
S718 | Phosphorylation | Uniprot | |
T735 | Phosphorylation | Q13043 (STK4) , P49759 (CLK1) , P41279 (MAP3K8) , Q9HAZ1 (CLK4) , Q13188 (STK3) , P33981 (TTK) | Uniprot |
T751 | Phosphorylation | Uniprot | |
K759 | Acetylation | Uniprot | |
T781 | Phosphorylation | Uniprot | |
S805 | Phosphorylation | Uniprot | |
S808 | Phosphorylation | Uniprot | |
S809 | Phosphorylation | Uniprot | |
T814 | Phosphorylation | Uniprot | |
T823 | Phosphorylation | Uniprot | |
T844 | Phosphorylation | Uniprot | |
T852 | Phosphorylation | Uniprot | |
T854 | Phosphorylation | Uniprot | |
S855 | Phosphorylation | Uniprot | |
S859 | Phosphorylation | Uniprot | |
S884 | Phosphorylation | Uniprot | |
S915 | Phosphorylation | Uniprot | |
S917 | Phosphorylation | Uniprot | |
S919 | Phosphorylation | Uniprot | |
T935 | Phosphorylation | Uniprot | |
S936 | Phosphorylation | Uniprot | |
S943 | Phosphorylation | Uniprot | |
S949 | Phosphorylation | Uniprot | |
T963 | Phosphorylation | Uniprot | |
S977 | Phosphorylation | Uniprot | |
Y1064 | Phosphorylation | Uniprot | |
Y1070 | Phosphorylation | Uniprot | |
S1106 | Phosphorylation | Uniprot | |
T1111 | Phosphorylation | Uniprot |
研究背景
Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717'. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).
Acetylated at Lys-711 by EP300 which promotes the cytoplasmic translocation.
Phosphorylation at Tyr-70 by members of the SRC family of kinases disrupts SH3 domain-based autoinhibitory interactions and intermolecular associations, such as that with ABI1, and also enhances kinase activity. Phosphorylation at Tyr-226 and Tyr-393 correlate with increased activity. DNA damage-induced activation of ABL1 requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation at Ser-569 has been attributed to a CDC2-associated kinase and is coupled to cell division (By similarity). Phosphorylation at Ser-618 and Ser-619 by PAK2 increases binding to CRK and reduces binding to ABI1. Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Phosphorylated by PRKDC (By similarity).
Polyubiquitinated. Polyubiquitination of ABL1 leads to degradation.
Cytoplasm>Cytoskeleton. Nucleus. Mitochondrion.
Note: Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity).
Nucleus membrane>Lipid-anchor.
Note: The myristoylated c-ABL protein is reported to be nuclear.
Widely expressed.
Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16, ITGB1 and HCK (By similarity). Interacts with STX17; probably phosphorylates STX17. Interacts with INPPL1/SHIP2. Interacts with the 14-3-3 proteins, YWHAB, YWHAE, YWHAG, YWHAH, SFN AND YWHAZ; the interaction with 14-3-3 proteins requires phosphorylation on Thr-735 and, sequesters ABL1 into the cytoplasm. Interacts with ABI1, ABI2, BCR, CRK, FGR, FYN, HCK, LYN, PSMA7 RAD9A, RAD51, RAD52, TP73 and WASF3. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Interacts (via SH3 domain) with CASP9; the interaction is direct and increases in the response of cells to genotoxic stress and ABL1/c-Abl activation. Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases. Interacts with TBX21 (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
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