Phospho-Chk2 (Thr68) Antibody - #AF3036

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产品: 磷酸化 Chk2 (Thr68) 抗体
货号: AF3036
描述: Rabbit polyclonal antibody to Phospho-Chk2 (Thr68)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: O96017
RRID: AB_2834325

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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Chk2 (Thr68) Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 68.
RRID:
AB_2834325
引用格式: Affinity Biosciences Cat# AF3036, RRID:AB_2834325.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDS 1; Cds1; Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; CHEK 2; Chek2; Chk 2; CHK2 checkpoint homolog (S. pombe); CHK2 checkpoint homolog; CHK2_HUMAN; hCds1; HuCds 1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2;

抗原和靶标

免疫原:

A synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68.

基因/基因ID:
CHEK2(11200)
描述:
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

文献引用

1). The activated ATM/p53 pathway promotes autophagy in response to oxidative stress-mediated DNA damage induced by Microcystin-LR in male germ cells. Ecotoxicology and Environmental Safety, 2021 (PubMed: 34715501) [IF=6.2]

Application: WB    Species: Mouse    Sample: GC-1 cells

Fig. 4. Changes of ATM and its downstream proteins in GC-1 cells and mouse testis. Expression and analysis of the related proteins in mouse testis (A) and in GC-1 cells (B). *p < 0.05 vs. the control group; #p < 0.05 vs. the corresponding MC-LR exposure group. All data were expressed as  ± SD (n = 3).
2). Critical role of checkpoint kinase 1 in spinal cord injury-induced motor dysfunction in mice. International immunopharmacology, 2024 (PubMed: 38917519) [IF=4.8]
3). Role of DNA damage in the progress of chronic tubule‑interstitial injury. Molecular Medicine Reports, 2020 (PubMed: 32626982) [IF=3.4]

Application: WB    Species: human    Sample: HK-2 cells

Figure 4.| - p21 knockdown reduces the DNA damage response in HK-2 cells. (A) Protein expression levels of p-ATM, ATM, p-Chk2, Chk2, rH2AX, p-p53 and p53 were analyzed using western blotting in cells transfected with shp21 or shCon with or without AA-induced injury.
4). FAK inhibitor PF-562271 inhibits the migration and proliferation of high-grade serous ovarian cancer cells through FAK and FAK mediated cell cycle arrest. Medical oncology (Northwood, London, England), 2023 (PubMed: 37382687) [IF=2.8]
5). Acrylamide-induced meiotic arrest of spermatocytes in adolescent mice by triggering excessive DNA strand breaks: Potential therapeutic effects of resveratrol. Human & experimental toxicology, 2023 (PubMed: 37550604) [IF=2.7]

Application: WB    Species: Mouse    Sample:

Figure 3. Effects of AA on the meiotic process of pachytene spermatocytes and the expression of meiotic DSB signaling proteins. (a) Representative immunofluorescence images of SYCP3 and γH2AX staining in the pachytene spermatocytes of the testis. (b) and (c) The expression levels of meiotic DSB signaling proteins (γH2AX, p-ATM and p-CHK2) in the testis and isolated spermatocytes, respectively. The data are expressed as the mean ± SD of three mice in each group for annotation B and expressed as the mean ± SE of three separate experiments with triplicate samples for annotation C. *p < 0.05, **p < 0.01, compared with the control group.

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