Phospho-CDK5 (Ser159) Antibody - #AF8366

Figure 1. Bacteria pathogens induce the expression of hemocyanin, SREBP, and ER stress proteins in Penaeus vannamei hepatopancreas. PvHMC mRNA and protein expression after challenge with (A) Vibrio parahaemolyticus and (B) Streptococcus iniae. PvSREBP mRNA and protein expression after challenge with (C) V. parahaemolyticus, and (D) S. iniae for 72 h. Protein expression levels of Bip, Xbp1s, and PvHMC after challenge with (E) V. parahaemolyticus, and (F) S. iniae for 0, 25, 48, and 72 h. The mRNA levels were quantified by qRT-PCR and normalized to those of EF1a mRNA, while protein levels were determined by Western blot using the indicated antibodies, with tubulin used as the loading control. Protein band intensity was analyzed using ImageJ and normalized relative to tubulin. Results are reported as mean ± SEM (n = 3). * p < 0.05, ** p < 0.01 vs. control (PBS). The immunoblots shown are representative of at least three independent experiments. PvHMC: hemocyanin; PvSREBP: sterol regulatory element binding protein homolog of Penaeus vannamei; Bip: binding-immunoglobulin protein; Xbp1s: X-box-binding protein; PBS: phosphate-buffered saline.