Phospho-beta Catenin (Ser552) Antibody - #AF8340

>>赠JD卡 进行中>>
(3)   (4)  
产品: 磷酸化 beta Catenin (Ser552) 抗体
货号: AF8340
描述: Rabbit polyclonal antibody to Phospho-beta Catenin (Ser552)
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P35222
RRID: AB_2840402

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-beta Catenin (Ser552) Antibody detects endogenous levels of beta Catenin only when phosphorylated at Ser552.
RRID:
AB_2840402
引用格式: Affinity Biosciences Cat# AF8340, RRID:AB_2840402.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Beta catenin; Beta-catenin; Cadherin associated protein; Catenin (cadherin associated protein), beta 1, 88kDa; Catenin beta 1; Catenin beta-1; CATNB; CHBCAT; CTNB1_HUMAN; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923; OTTHUMP00000162082; OTTHUMP00000165222; OTTHUMP00000165223; OTTHUMP00000209288; OTTHUMP00000209289;

抗原和靶标

免疫原:

A synthesized peptide derived from human Catenin-β around the phosphorylation site of Ser552.

基因/基因ID:
CTNNB1(1499)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Zebrafish cox17 modulates primitive erythropoiesis via regulation of mitochondrial metabolism to facilitate hypoxia tolerance. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2022 (PubMed: 36208295) [IF=4.4]
2). Calycosin attenuates pulmonary fibrosis by the epithelial-mesenchymal transition repression upon inhibiting the AKT/GSK3β/β-catenin signaling pathway. ACTA HISTOCHEMICA, 2021 (PubMed: 34217047) [IF=2.3]

Application: WB    Species: Mice    Sample: lung tissues

Fig. 3. Calycosin repressed the activity of the AKT/GSK3β/β-catenin signaling pathway induced by bleomycin. One day after 5 mg/kg BLM instillation, mice were orally adminis- trated with 7 mg/kg or 14 mg/kg CA once a day for three weeks. (a) The protein levels of p-AKT, AKT, p-GSK3β, GSK3β, p-β-catenin, and β-cat- enin in lung tissues of mice. (b) Immunohisto- chemistry staining of β-catenin. Red arrow: the expression of β-catenin in nucleus. Scale bar =50 μ m. Data were expressed as mean ± SD. n = 6 in each group. **p < 0.01 vs. control; #p < 0.05, ##p < 0.01 vs. BLM.
3). Zebrafish ELL-associated factors Eaf1/2 modulate erythropoiesis via regulating gata1a expression and WNT signaling to facilitate hypoxia tolerance. Cell regeneration (London, England), 2023 (PubMed: 37002435)

Application: WB    Species: Zebrafish    Sample:

Fig. 5 Effects of eaf1/2 deficiency on WNT/β-catenin signaling during fish erythrogenesis. A Protein levels of P-β-catenin ser 552 and β-catenin in eaf1−/−, eaf2−/−, and WT embryos at 16 hpf and 24 hpf (A1-A2), respectively, and quantitative analysis of protein level in each sample (A3-A4). B Endogenous WNT/β-catenin signaling activities in eaf1−/−, eaf2−/−, and WT embryos at 16 hpf and 24 hpf, respectively. One-cell stage embryos were injected with TopFlash (as a reporter) and TK-renilla (as an internal control) together, and the injected embryos were collected for assays at 16 hpf and 24 hpf, respectively. C Double staining of gata1aDsRed+ and β-Catetin, in the control and embryos injected with eaf1-MO or eaf2-MO at 24 hpf (C1-C15), and quantification of β-Catenin immunofluorescence intensities in gata1aDsRed+ cells (C16), and C13-C15 show the magnified views of C10-C12, respectively. D Chromatin immunoprecipitation (ChIP) analysis of the binding enrichment of protein TCF4 on the promoter of gene scl (D1) and gene lmo2 (D2) in eaf1−/− and eaf2−/− embryonic cells at 24 hpf, respectively, with anti- IgG used as a negative control. E WISH analysis of the expression of gata1a and hbbe3 in eaf1−/−, eaf2−/−, WT embryos and the corresponding groups treated with Wnt activator BIO at 24 hpf (E1-E12), and statistical analysis of WISH results (E13, E14). Each experiment was repeated at least three times, with similar results for two or three replicates, and a representative result was shown. Data are mean ± SD. C1-C15, E1-E12, lateral view, anterior to the left. *P 

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.