| 产品: | 磷酸化 XPA (Ser196) 抗体 |
| 货号: | AF8335 |
| 描述: | Rabbit polyclonal antibody to Phospho-XPA (Ser196) |
| 应用: | WB |
| 反应: | Human, Mouse, Monkey |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 40kDa; 31kD(Calculated). |
| 蛋白号: | P23025 |
| RRID: | AB_2840397 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8335, RRID:AB_2840397.
展开/折叠
DNA repair protein complementing XP A cells; DNA repair protein complementing XP-A cells; DNA repair protein complementing XPA cells; Excision repair controlling; Xeroderma pigmentosum 1; Xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A complementing protein; Xeroderma pigmentosum group A-complementing protein; XP 1; XP1; xpa; XPA_HUMAN; Xpac;
抗原和靶标
- P23025 XPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P23025 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K63 | Sumoylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K86 | Sumoylation | Uniprot | |
| K141 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K145 | Sumoylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | P78527 (PRKDC) , Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| S196 | Phosphorylation | P78527 (PRKDC) , Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| K204 | Ubiquitination | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| Y257 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot |
研究背景
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage.
Ubiquitinated by HERC2 leading to degradation by the proteasome.
Nucleus.
Expressed in various cell lines and in skin fibroblasts.
Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2.
Belongs to the XPA family.
研究领域
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
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