ILKAP Antibody - #AF0526

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产品: ILKAP 抗体
货号: AF0526
描述: Rabbit polyclonal antibody to ILKAP
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 45kDa; 43kD(Calculated).
蛋白号: Q9H0C8
RRID: AB_2834319

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(82%), Horse(91%), Rabbit(82%), Dog(82%)
克隆:
Polyclonal
特异性:
ILKAP Antibody detects endogenous levels of total ILKAP.
RRID:
AB_2834319
引用格式: Affinity Biosciences Cat# AF0526, RRID:AB_2834319.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp434J2031; FLJ10181; ILKAP; ILKAP_HUMAN; ILKAP2; ILKAP3; Integrin linked kinase associated phosphatase; Integrin linked kinase associated serine/threonine phosphatase 2C; Integrin linked kinase associated serine/threonine phosphatase; Integrin-linked kinase-associated serine/threonine phosphatase 2C; MGC4846; PP2C DELTA; Protein phosphatase 2c delta isozyme;

抗原和靶标

免疫原:
Uniprot:

Q9H0C8(ILKAP_HUMAN) >>Visit HPA database.

基因/基因ID:
ILKAP(80895)
表达:
Q9H0C8 ILKAP_HUMAN:

Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.

描述:
ILKAP a protein serine/threonine phosphatase of the PP2C family. Can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. Alternatively spliced transcript variants encoding distinct isoforms have been described.
序列:
MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
91
Horse
91
Bovine
82
Dog
82
Rabbit
82
Sheep
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9H0C8 作为底物

Site PTM Type Enzyme
M1 Acetylation
S13 Phosphorylation
T81 Phosphorylation
S82 Phosphorylation
K86 Acetylation
K95 Acetylation
K161 Acetylation
K161 Ubiquitination
K185 Ubiquitination
C190 S-Nitrosylation
K196 Ubiquitination
K204 Methylation
K204 Ubiquitination
K210 Acetylation
K210 Ubiquitination
K250 Ubiquitination
K258 Ubiquitination
T263 Phosphorylation
Y265 Phosphorylation
K273 Ubiquitination
S290 Phosphorylation
Y298 Phosphorylation
T304 Phosphorylation
S305 Phosphorylation
R319 Methylation
R369 Methylation
R377 Methylation

研究背景

功能:

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.

亚基结构:

Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner.

蛋白家族:

Belongs to the PP2C family.

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