产品: BHLHB3 抗体
货号: AF0442
描述: Rabbit polyclonal antibody to BHLHB3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Dog
分子量: 50kDa; 50kD(Calculated).
蛋白号: Q9C0J9
RRID: AB_2834318

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(92%), Dog(92%)
克隆:
Polyclonal
特异性:
BHLHB3 Antibody detects endogenous levels of total BHLHB3.
RRID:
AB_2834318
引用格式: Affinity Biosciences Cat# AF0442, RRID:AB_2834318.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Basic helix-loop-helix domain containing class B3; BHE41_HUMAN; bHLH protein DEC2; bHLHB3; bHLHe41; Class B basic helix loop helix protein 3; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; DEC2; Differentially expressed in chondrocytes protein 2; Enhancer of split and hairy related protein 1; Enhancer-of-split and hairy-related protein 1; hDEC2; Moderately similar to basic-helix loop helix protein M.musculus; SHARP-1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9C0J9 BHE41_HUMAN:

Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.

描述:
BHLHB3 May be a transcriptional repressor that represses both basal and activated transcription. Homodimerize.
序列:
MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAKEVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKLDSRGGGSGGGPGGGAAAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLPFCFLSPSAAAAYVQPFLDKSGLEKYLYPAAAAAPFPLLYPGIPAPAAAAAAAAAAAAAAAAFPCLSSVLSPPPEKAGAAAATLLPHEVAPLGAPHPQHPHGRTHLPFAGPREPGNPESSAQEDPSQPGKEAP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
92
Dog
92
Pig
91
Chicken
58
Zebrafish
50
Horse
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9C0J9 作为底物

Site PTM Type Enzyme
K42 Acetylation
K46 Acetylation
K76 Ubiquitination
S122 Phosphorylation
S151 Phosphorylation
K210 Sumoylation
T236 Phosphorylation
K266 Sumoylation
S274 Phosphorylation

研究背景

功能:

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.

亚基结构:

Homodimer (By similarity). Heterodimer with BHLHE40/DEC1. Interacts with CIART and ARNTL/BMAL1 (By similarity). Interacts with RXRA. Interacts with NR0B2 and HNF1A (By similarity).

研究领域

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

文献引用

1). BHLHE41 Overexpression Alleviates the Malignant Behavior of Colon Cancer Cells Induced by Hypoxia via Modulating HIF-1α/EMT Pathway. Gastroenterology Research and Practice, 2022 (PubMed: 35615737) [IF=2.0]

Application: WB    Species: Human    Sample: colon cancer (CC) cells

Figure 1 BHLHE41 was low expressed in HCT116 and Lovo cells. (a) and (b) The level of BHLHE41 in colon cancer (CC) cells and normal cells was tested by Western blot and qRT-PCR. All experiments have been performed in triplicate, and data were expressed as mean ± standard deviation (SD).

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