(1)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0442, RRID:AB_2834318.
展开/折叠
Basic helix-loop-helix domain containing class B3; BHE41_HUMAN; bHLH protein DEC2; bHLHB3; bHLHe41; Class B basic helix loop helix protein 3; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; DEC2; Differentially expressed in chondrocytes protein 2; Enhancer of split and hairy related protein 1; Enhancer-of-split and hairy-related protein 1; hDEC2; Moderately similar to basic-helix loop helix protein M.musculus; SHARP-1;
抗原和靶标
Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
- Q9C0J9 BHE41_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAKEVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKLDSRGGGSGGGPGGGAAAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLPFCFLSPSAAAAYVQPFLDKSGLEKYLYPAAAAAPFPLLYPGIPAPAAAAAAAAAAAAAAAAFPCLSSVLSPPPEKAGAAAATLLPHEVAPLGAPHPQHPHGRTHLPFAGPREPGNPESSAQEDPSQPGKEAP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9C0J9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K42 | Acetylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K210 | Sumoylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K266 | Sumoylation | Uniprot | |
| S274 | Phosphorylation | Uniprot |
研究背景
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).
Nucleus.
Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
Homodimer (By similarity). Heterodimer with BHLHE40/DEC1. Interacts with CIART and ARNTL/BMAL1 (By similarity). Interacts with RXRA. Interacts with NR0B2 and HNF1A (By similarity).
研究领域
· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)
文献引用
Application: WB Species: Human Sample: colon cancer (CC) cells
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