RHOBTB1/2 Antibody - #AF0450

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产品: RHOBTB1/2 抗体
货号: AF0450
描述: Rabbit polyclonal antibody to RHOBTB1/2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse
分子量: 83kDa; 83kD,79kD(Calculated).
蛋白号: Q9BYZ6 | O94844
RRID: AB_2834314

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 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
RHOBTB1/2 Antibody detects endogenous levels of total RHOBTB1/2.
RRID:
AB_2834314
引用格式: Affinity Biosciences Cat# AF0450, RRID:AB_2834314.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DBC 2; Deleted in breast cancer 2; Deleted in breast cancer 2 gene protein; p83; RHBT2_HUMAN; Rho related BTB domain containing 2; Rho related BTB domain containing protein 2; Rho-related BTB domain-containing protein 2; RHOBTB 2; Rhobtb2; 1700008H16Rik; 3110048G13Rik; AI173445; AI573858; AV350930; KIAA0740; MGC107538; MGC33059; MGC33841; OTTHUMP00000019662; OTTHUMP00000019663; RHBT1_HUMAN; Rho related BTB domain containing 1; Rho related BTB domain containing protein 1; Rho-related BTB domain-containing protein 1; Rhobtb1;

抗原和靶标

免疫原:

A synthesized peptide derived from human RHBT2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9BYZ6(RHBT2_HUMAN) >>Visit HPA database.

O94844(RHBT1_HUMAN) >>Visit HPA database.

基因/基因ID:
RHOBTB1(9886) | RHOBTB2(23221)
表达:
Q9BYZ6 RHBT2_HUMAN:

Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.

O94844 RHBT1_HUMAN:

Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.

描述:
RhoBTB2 Belongs to the small GTPase superfamily. Rho family.
序列:
MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSEPGGTHPEDHQGHSDQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTYKSRLMVVVKMDSSIQPGPFRAVLKYLYTGELDENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIILANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLKRQPKRRWLFWNSPSSPSSSAASSSSPSSSSAVV

MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSNKSLVEALGLEAEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYTGQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFKLDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELIALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA

研究背景

组织特异性:

Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.

亚基结构:

Interacts with HSP90AA1 and HSP90AB1. Interacts with CUL3.

蛋白家族:

Belongs to the small GTPase superfamily. Rho family.

组织特异性:

Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.

蛋白家族:

Belongs to the small GTPase superfamily. Rho family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

文献引用

1). LY86 facilitates ox-LDL-induced lipid accumulation in macrophages by upregulating SREBP2/HMGCR expression. BMC cardiovascular disorders, 2024 (PubMed: 38822281) [IF=2.0]

Application: WB    Species: human    Sample: THP-1 cells

Fig. 4 LY86 effectively attenuates ox-LDL-induced lipid accumulation in THP-1 macrophages. (A) Representative images of oil red O staining in THP-1 cells (×40 magnification). (B, C) The cropped blot and quantification of intracellular HMGCR, SREBP2, GRP78, and LY86 after 24-hour knockdown treatment with si-LY86 followed by 24-hour stimulation with ox-LDL; groups 1–4 represent the following conditions: 1 si-NC; 2 si-NC + ox-LDL; 3 si-LY86; and 4 si-LY86 + ox-LDL. Data are presented as mean ± SD (n = 3); *P

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