Nucleophosmin Antibody - #AF0441

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产品: Nucleophosmin 抗体
货号: AF0441
描述: Rabbit polyclonal antibody to Nucleophosmin
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P06748
RRID: AB_2834312

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Nucleophosmin Antibody detects endogenous levels of total Nucleophosmin.
RRID:
AB_2834312
引用格式: Affinity Biosciences Cat# AF0441, RRID:AB_2834312.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B23; MGC104254; NO38; NPM; NPM_HUMAN; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; nucleophosmin nucleoplasmin family member 1; Nucleophosmin/nucleoplasmin family member 1; Numatrin; OTTHUMP00000161024; OTTHUMP00000161025; OTTHUMP00000223397; OTTHUMP00000223398;

抗原和靶标

免疫原:

A synthesized peptide derived from human Nucleophosmin, corresponding to a region within N-terminal amino acids.

基因/基因ID:
NPM1(4869)
描述:
NPM1 a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

文献引用

1). XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36791564) [IF=6.9]

Application: WB    Species: human    Sample: HepaRG cells

Fig. 3. XPO1 interacts with NPM1. (A) Immunoaffinity purification and mass spectrometry analysis of XPO1-associated proteins in Huh7/SR cells. The proteins with NES structure and interacting with XPO1 are displayed. (B) and (C) Immunoblotting (IB) analysis of WCL and anti-XPO1 immunoprecipitates (IPs) derived from Huh7 and HepG2 cells. Immunoglobulin G (IgG) served as a negative control. (D) Molecular docking of XPO1 and NPM1. (E) Schematic representation of the deletion mutants of NPM1 with the key to domain organization. (F) HA-NPM1 or its mutants NPM11–119, NPM11–147 and NPM1119–294 were individually transfected into HepaRG cells along with Flag-XPO1. Cell lysates were immunoprecipitated with the anti-Flag antibody and then immunoblotted with the indicated antibodies. n = 3. (G) R analysis of the correlation between XPO1 and NPM1 in TCGA HCC dataset. (H) IB analysis of WCL derived from HepaRG cells transfected with XPO1-OE plasmid. Blots are representative of 3 experiments. (I) HepaRG/XPO1-OE and HepaRG/Vector cells were treated with 10 μM MG132 for the indicated times. The total cell lysates were then analyzed by Western blot with the indicated antibodies. n = 3. * * P 

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