Phospho-nNOS (Ser1417) Antibody - #AF8231

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产品: 磷酸化 nNOS (Ser1417) 抗体
货号: AF8231
描述: Rabbit polyclonal antibody to Phospho-nNOS (Ser1417)
应用: WB IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Rabbit, Dog, Chicken, Xenopus
蛋白号: P29475
RRID: AB_2840293

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-nNOS (Ser1417) Antibody detects endogenous levels of nNOS only when phosphorylated at Ser1417.
RRID:
AB_2840293
引用格式: Affinity Biosciences Cat# AF8231, RRID:AB_2840293.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2310005C01Rik; BNOS; Constitutive NOS; EC 1.14.13.39; IHPS 1; IHPS1; N-NOS; NC-NOS; neuronal Nitric Oxide Synthase; Neuronal NOS; Nitric oxide synthase , neuronal, included; Nitric oxide synthase 1 (neuronal); Nitric oxide synthase 1; Nitric oxide synthase, brain; Nitric oxide synthase, penile neuronal, included; NNOS; NO; NOS 1; NOS; NOS type I; NOS-I; NOS1; NOS1_HUMAN; Peptidyl-cysteine S-nitrosylase NOS1;

抗原和靶标

免疫原:

A synthesized peptide derived from human nNOS around the phosphorylation site of Ser1417.

基因/基因ID:
NOS1(4842)

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Salivary secretion.

文献引用

1). Nitro-oleic acid ameliorates erectile dysfunction in a streptozotocin-induced rat model of diabetes by inhibiting oxidative stress and apoptosis and activating the NO/cGMP pathway. Asian Journal of Andrology, 2023 (PubMed: 37695220) [IF=3.0]

Application: IF/ICC    Species: Rat    Sample: corpus cavernosum tissue

Figure 3 Changes in the NO/cGMP pathway within the corpus cavernosum region of the four groups. (a) NO levels were determined using assay kits. Concentrations of (b) cGMP and (c) ADMA in the corpus cavernosum region as determined using ELISA. The expression levels of (d) p-eNOS and (e) p-nNOS were determined using immunofluorescence. Scale bars = 50 µm. Bar graph illustrating (f) eNOS and (g) nNOS concentrations in the corpus cavernosum region. n = 5 per group. All data are presented as the mean ± standard error of the mean. ***P < 0.001, ****P < 0.0001, the indicated group vs the control group; #P < 0.05, ##P < 0.01, the indicated group vs the DMED group. NO2-OA: nitro-oleic acid; DMED: diabetes mellitus erectile dysfunction; p-eNOS: phospho-endothelial nitric oxide synthase; p-nNOS: phospho-neuronal nitric oxide synthase; ADMA: asymmetric dimethylarginine; NO: nitric oxide; cGMP: 3’,5’-cyclic guanosine monophosphate; DAPI: 4’,6-diamidino-2-phenylindole; ELISA: enzyme-linked immunosorbent assay.
2). Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes. FEBS open bio, 2025 (PubMed: 39961145) [IF=2.8]

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