Phospho-FGFR1/2/3/4 (Tyr653+Tyr654) Antibody - #AF8210

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产品: 磷酸化 FGFR1/2/3/4 (Tyr653+Tyr654) 抗体
货号: AF8210
描述: Rabbit polyclonal antibody to Phospho-FGFR1/2/3/4 (Tyr653+Tyr654)
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P11362 | P21802 | P22607 | P22455
RRID: AB_2840272

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Phospho-FGFR1/2/3/4 (Tyr653+Tyr654) Antibody detects endogenous levels of FGFR1/2/3/4 only when phosphorylated at Tyr653+Tyr654.
RRID:
AB_2840272
引用格式: Affinity Biosciences Cat# AF8210, RRID:AB_2840272.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Basic fibroblast growth factor receptor 1; bFGF-R-1; BFGFR; CD331; CEK; FGFBR; FGFR 1; FGFR-1; FGFR1; FGFR1/PLAG1 fusion; FGFR1_HUMAN; fibroblast growth factor receptor 1; FLG; FLT-2; FLT2; Fms-like gene; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; bacteria-expressed kinase; BBDS; BEK; BEK fibroblast growth factor receptor; BFR1; CD332; CD332 antigen; CEK3; CFD1; Craniofacial dysostosis 1; ECT1; FGF receptor; FGFR 2; FGFR-2; Fgfr2; FGFR2_HUMAN; Fibroblast growth factor receptor 2; Hydroxyaryl protein kinase; Jackson Weiss syndrome; JWS; K SAM; K-sam; Keratinocyte growth factor receptor 2; Keratinocyte growth factor receptor; KGFR; KSAM; protein tyrosine kinase, receptor like 14; soluble FGFR4 variant 4; TK14; TK25; ACH; CD 333; CD333; CD333 antigen; CEK 2; CEK2; FGFR 3; FGFR-3; FGFR3; FGFR3_HUMAN; Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism); Fibroblast growth factor receptor 3; Heparin binding growth factor receptor; HSFGFR3EX; Hydroxyaryl protein kinase; JTK 4; JTK4; MFR 3; SAM 3; Tyrosine kinase JTK 4; Tyrosine kinase JTK4; Z FGFR 3; CD 334; CD334; CD334 antigen; fc13h 10; fc13h10; Fgfr 4; FGFR-4; Fgfr4; FGFR4_HUMAN; Fibroblast growth factor receptor 4; Hydroxyaryl protein kinase; JTK 2; JTK2; MGC20292; Protein tyrosine kinase; TKF; Tyrosine kinase related to fibroblast growth factor receptor; Tyrosylprotein kinase;

抗原和靶标

免疫原:

A synthesized peptide derived from human FGFR1/2/3/4 around the phosphorylation site of Tyr653+Tyr654.

基因/基因ID:
FGFR1(2260) | FGFR2(2263) | FGFR4(2264) | FGFR3(2261)

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

文献引用

1). A novel small molecule RK-019 inhibits FGFR2-amplification gastric cancer cell proliferation and induces apoptosis in vitro and in vivo. Frontiers in Pharmacology, 2022 (PubMed: 36210834) [IF=5.6]
2). Vascular Adventitial Fibroblasts-Derived FGF10 Promotes Vascular Smooth Muscle Cells Proliferation and Migration in vitro and the Neointima Formation in vivo. Journal of Inflammation Research, 2021 (PubMed: 34079328) [IF=4.5]

Application: IHC    Species: Rat    Sample: VSMCs

Figure 5 The activities of FGFR2 in VSMCs in vivo and in vitro. (A and C) Representative IHC images of P-FGFR2 staining (A) and its quantitative analysis (C) in rat LCA sections harvested at indicated times after balloon-injury. Scale bar=50μm, n=6. (B and D) Representative immunofluorescence images of P-FGFR2 staining (B) and its quantitative analysis (D) in cultured VSMCs under conditions as indicated. Scale bar=20μm, n=3. (E–G) The protein expression of P-FGFR2 in co-cultured VSMCs under conditions described in Figure 3D were analyzed by WB (E) and its quantitative analyses (F and G), n=3. *p<0.05; **p<0.01 vs Ctl. # p<0.05; ## p<0.01 between two groups as marked.

Application: IF/ICC    Species: Rat    Sample: VSMCs

Figure 5 The activities of FGFR2 in VSMCs in vivo and in vitro. (A and C) Representative IHC images of P-FGFR2 staining (A) and its quantitative analysis (C) in rat LCA sections harvested at indicated times after balloon-injury. Scale bar=50μm, n=6. (B and D) Representative immunofluorescence images of P-FGFR2 staining (B) and its quantitative analysis (D) in cultured VSMCs under conditions as indicated. Scale bar=20μm, n=3. (E–G) The protein expression of P-FGFR2 in co-cultured VSMCs under conditions described in Figure 3D were analyzed by WB (E) and its quantitative analyses (F and G), n=3. *p<0.05; **p<0.01 vs Ctl. # p<0.05; ## p<0.01 between two groups as marked.

Application: WB    Species: Rat    Sample: VSMCs

Figure 2 PDGF differentially regulates expression of FGF10 and FGFR2 in VECs, VSMCs and VAFs. VECs, VSMCs and VAFs were isolated from rat aorta and cultured. (A) Morphological features of VECs, VSMCs and VAFs in primary cultures. Bar=200μm. (B) Immunofluorescence staining of CD31, α-SMA and Vimentin in three types of cells. Nuclei were stained with DAPI. Bar=20μm. (C and D) Representative WB images (C) and summaries (D) of FGF10 and FGFR2 protein expressions in three types of cells with or with put treatment of PDGF (20ng/mL). n=3. *p<0.05 and **p<0.01. Abbreviation: ns, no significance.
3). Dapagliflozin promotes browning of white adipose tissue through the FGFR1-LKB1-AMPK signaling pathway. Molecular biology reports, 2024 (PubMed: 38644407) [IF=2.6]

Application: WB    Species: Mouse    Sample: 3T3-L1 cells

Fig. 5 Effects of DAPA on AMPK pathway in 3T3-L1 cells. After treatment with PD, Pim1, CC, and treatment with DAPA, heat shock protein levels and the protein expression of p-FGFR1, p-LKB1, and p-AMPK were measured (A). Heat shock protein expression and the protein expression of p-LKB1, and p-AMPK were also measured (B). Heat shock protein expression and the protein expression of p-AMPK were also measured (C). Data are presented as mean ± SD; **P 

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