MAP3K3 Antibody - #AF0449

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产品: MAP3K3 抗体
货号: AF0449
描述: Rabbit polyclonal antibody to MAP3K3
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 71kDa; 71kD(Calculated).
蛋白号: Q99759
RRID: AB_2834305

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)
克隆:
Polyclonal
特异性:
MAP3K3 Antibody detects endogenous levels of total MAP3K3.
RRID:
AB_2834305
引用格式: Affinity Biosciences Cat# AF0449, RRID:AB_2834305.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

M3K3_HUMAN; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; Mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3;

抗原和靶标

免疫原:
Uniprot:

Q99759(M3K3_HUMAN) >>Visit HPA database.

基因/基因ID:
MAP3K3(4215)
描述:
This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
序列:
MDEQEALNSIMNDLVALQMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
91
Chicken
82
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99759 作为底物

Site PTM Type Enzyme
K102 Ubiquitination
S130 Phosphorylation
S131 Phosphorylation
S145 Phosphorylation
S147 Phosphorylation
Y155 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation O00141 (SGK1)
S168 Phosphorylation
S169 Phosphorylation
S175 Phosphorylation
S176 Phosphorylation
S194 Phosphorylation
S234 Phosphorylation
S237 Phosphorylation
S250 Phosphorylation
T263 Phosphorylation
Y266 Phosphorylation
T274 Phosphorylation
Y275 Phosphorylation
Y288 Phosphorylation
S289 Phosphorylation
T294 Phosphorylation
S312 Phosphorylation
S314 Phosphorylation
S316 Phosphorylation
T317 Phosphorylation
S337 Phosphorylation O00141 (SGK1)
S340 Phosphorylation
S345 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
K366 Ubiquitination
K391 Ubiquitination
K404 Ubiquitination
K417 Ubiquitination
K456 Ubiquitination
S464 Phosphorylation
K491 Ubiquitination
K504 Ubiquitination
K512 Ubiquitination
T516 Phosphorylation
S520 Phosphorylation
S526 Phosphorylation Q99759 (MAP3K3)
T528 Phosphorylation
T530 Phosphorylation

翻译修饰 - Q99759 作为激酶

Substrate Site Source
P46734 (MAP2K3) S218 Uniprot
P53805 (RCAN1) S163 Uniprot
P53805 (RCAN1) S167 Uniprot
Q99759-1 (MAP3K3) S526 Uniprot
Q99759-2 (MAP3K3) S557 Uniprot

研究背景

功能:

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

翻译修饰:

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.

亚基结构:

Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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