产品: 磷酸化 CGK2 (Ser126) 抗体
货号: AF8184
描述: Rabbit polyclonal antibody to Phospho-CGK2 (Ser126)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 87 KD; 87kD(Calculated).
蛋白号: Q13237
RRID: AB_2840246

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
克隆:
Polyclonal
特异性:
Phospho-CGK2 (Ser126) Antibody detects endogenous levels of CGK2 only when phosphorylated at Ser126.
RRID:
AB_2840246
引用格式: Affinity Biosciences Cat# AF8184, RRID:AB_2840246.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CGK 2; cGK2; cGKII; cGMP dependent protein kinase 2; cGMP-dependent protein kinase 2; cGMP-dependent protein kinase II; KGP2_HUMAN; PRKG 2; PRKG2; PRKGR2; Protein kinase, cGMP dependent, type II; Type II cGMP dependent protein kinase;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q13237 KGP2_HUMAN:

Highly concentrated in brain, lung and intestinal mucosa.

序列:
MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Zebrafish
90
Chicken
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13237 作为底物

Site PTM Type Enzyme
G2 Myristoylation
S11 Phosphorylation
S97 Phosphorylation Q13237 (PRKG2)
T109 Phosphorylation
S110 Phosphorylation Q13237 (PRKG2)
S114 Phosphorylation Q13237 (PRKG2)
S117 Phosphorylation Q13237 (PRKG2)
S126 Phosphorylation Q13237 (PRKG2)
T131 Phosphorylation
K157 Ubiquitination
K165 Sumoylation
K165 Ubiquitination
Y306 Phosphorylation
Y307 Phosphorylation
S431 Phosphorylation
S445 Phosphorylation Q13237 (PRKG2)
S448 Phosphorylation
K521 Methylation

翻译修饰 - Q13237 作为激酶

Substrate Site Source
P04626 (ERBB2) T686 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P04792 (HSPB1) T143 Uniprot
P29474 (NOS3) T495 Uniprot
P29474 (NOS3) S633 Uniprot
P29474 (NOS3) S1177 Uniprot
P30307 (CDC25C) S216 Uniprot
P32929 (CTH) S377 Uniprot
P42261 (GRIA1) S863 Uniprot
P48436 (SOX9) S181 Uniprot
P49675 (STAR) S56 Uniprot
P49675 (STAR) S57 Uniprot
P49675 (STAR) S100 Uniprot
P49841 (GSK3B) S9 Uniprot
P50552 (VASP) S239 Uniprot
P53350 (PLK1) S137 Uniprot
P55211 (CASP9) S196 Uniprot
Q01970-1 (PLCB3) S26 Uniprot
Q01970-1 (PLCB3) S1105 Uniprot
Q03393 (PTS) S19 Uniprot
Q12778 (FOXO1) S319 Uniprot
Q13237 (PRKG2) S97 Uniprot
Q13237 (PRKG2) S110 Uniprot
Q13237 (PRKG2) S114 Uniprot
Q13237 (PRKG2) S117 Uniprot
Q13237 (PRKG2) S126 Uniprot
Q13237 (PRKG2) S445 Uniprot
Q14847-1 (LASP1) S61 Uniprot
Q14847 (LASP1) S146 Uniprot
Q15418 (RPS6KA1) S380 Uniprot
Q9UL51 (HCN2) S668 Uniprot

研究背景

功能:

Crucial regulator of intestinal secretion and bone growth (By similarity). Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (By similarity). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (By similarity). Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).

翻译修饰:

Myristoylation mediates membrane localization.

细胞定位:

Apical cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly concentrated in brain, lung and intestinal mucosa.

亚基结构:

Interacts with GRIA1/GLUR1.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

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