KSR2 Antibody - #AF0688

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产品: KSR2 抗体
货号: AF0688
描述: Rabbit polyclonal antibody to KSR2
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 108kDa; 108kD(Calculated).
蛋白号: Q6VAB6
RRID: AB_2834291

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Zebrafish(91%), Bovine(100%), Horse(91%), Sheep(91%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
KSR2 Antibody detects endogenous levels of total KSR2.
RRID:
AB_2834291
引用格式: Affinity Biosciences Cat# AF0688, RRID:AB_2834291.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ25965; hKSR 2; hKSR2; Kinase suppressor of Ras 2; KSR 2; Ksr2; KSR2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q6VAB6(KSR2_HUMAN) >>Visit HPA database.

基因/基因ID:
KSR2(283455)
表达:
Q6VAB6 KSR2_HUMAN:

Mainly expressed in brain and kidney.

描述:
KSR2 Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Interacts with MAP2K1, MAP3K8, MAPK, RAS and RAF. 2 isoforms of the human protein are produced by alternative splicing.
序列:
MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
91
Horse
91
Sheep
91
Zebrafish
91
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q6VAB6 作为底物

Site PTM Type Enzyme
S34 Phosphorylation
K44 Ubiquitination
T260 Phosphorylation
T264 Phosphorylation
T269 Phosphorylation
T270 Phosphorylation
T272 Phosphorylation
T276 Phosphorylation
K281 Acetylation
T290 Phosphorylation
S294 Phosphorylation
T306 Phosphorylation
S311 Phosphorylation
S313 Phosphorylation
S347 Phosphorylation
S394 Phosphorylation
S399 Phosphorylation
S474 Phosphorylation
S490 Phosphorylation
T497 Phosphorylation
Y566 Phosphorylation
Y794 Phosphorylation
T802 Phosphorylation
S808 Phosphorylation
K923 Ubiquitination
S939 Phosphorylation

翻译修饰 - Q6VAB6 作为激酶

Substrate Site Source
Q02750 (MAP2K1) S24 Uniprot
Q02750 (MAP2K1) S72 Uniprot

研究背景

功能:

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

翻译修饰:

Phosphorylated on Ser-474 by MARK3.

细胞定位:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mainly expressed in brain and kidney.

亚基结构:

Heterodimerizes (via N-terminus) with BRAF (via N-terminus) in a MAP2K1/MEK1-dependent manner. Interacts with BRAF; this increases the low intrinsic protein kinase activity of KSR2. Interacts with MAP2K1, forming a heterodimer that can dimerize to form a heterotetramer. Interacts with MAP3K8, MAPK, RAS and RAF.

蛋白家族:

The protein kinase domain is predicted to be catalytically inactive and seems to have very low intrinsic kinase activity. This low kinase activity can be increased by interaction with BRAF.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

研究领域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

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