Phospho-CFTR (Ser737) Antibody - #AF8042

Fig. 2. Treatment with ivacaftor prevents the decrease of CFTR and the increase of p-CFTR (S737) after noise exposure in OHCs. (A) Representative images show immunolabeling for CFTR (red) and p-CFTR (S737, green) in OHC cytosol and nuclei in the upper basal turn measured 3 h after noise exposure. The enlarged OHCs allow for better visualization of the punctate labeling for CFTR and p-CFTR (S373). These images are representative of 3 mice in each group. Phalloidin (white) and DAPI (blue) were used as counterstaining for visualization of OHC cytoskeletal structure and nuclei, respectively. DMSO (control, solvent of ivacaftor); IVA (ivacaftor). Scale bar = 10 μm. (B–E) Semi-quantification of immunolabeling in grayscale of CFTR and p-CFTR (S737) in the OHC cytosol and nuclei shows that treatment with ivacaftor significantly prevents noise-decreased CFTR and increased p-CFTR (S737). Data are presented as means + SD, n = 3, *p < 0.05, **p < 0.01. (F) Representative immunoblots reveal a decrease in total CFTR expression and increase in p-CFTR (S737) in whole cochlear homogenates 3 h after noise exposure. These blots are representative of 3 repetitions with two cochleae per sample. GADPH serves as loading control. (G–I) Quantification of CFTR and p-CFTR (S737) band densities shows a significant decrease in CFTR levels (G), an increase in p-CFTR (S737) levels (H), and an increase in the ratio of p-CFTR (S737) to CFTR (I) in the PTS noise group compared to controls. Data are presented as means + SD, n = 3, **p < 0.01.

Fig. 2. Treatment with ivacaftor prevents the decrease of CFTR and the increase of p-CFTR (S737) after noise exposure in OHCs. (A) Representative images show immunolabeling for CFTR (red) and p-CFTR (S737, green) in OHC cytosol and nuclei in the upper basal turn measured 3 h after noise exposure. The enlarged OHCs allow for better visualization of the punctate labeling for CFTR and p-CFTR (S373). These images are representative of 3 mice in each group. Phalloidin (white) and DAPI (blue) were used as counterstaining for visualization of OHC cytoskeletal structure and nuclei, respectively. DMSO (control, solvent of ivacaftor); IVA (ivacaftor). Scale bar = 10 μm. (B–E) Semi-quantification of immunolabeling in grayscale of CFTR and p-CFTR (S737) in the OHC cytosol and nuclei shows that treatment with ivacaftor significantly prevents noise-decreased CFTR and increased p-CFTR (S737). Data are presented as means + SD, n = 3, *p < 0.05, **p < 0.01. (F) Representative immunoblots reveal a decrease in total CFTR expression and increase in p-CFTR (S737) in whole cochlear homogenates 3 h after noise exposure. These blots are representative of 3 repetitions with two cochleae per sample. GADPH serves as loading control. (G–I) Quantification of CFTR and p-CFTR (S737) band densities shows a significant decrease in CFTR levels (G), an increase in p-CFTR (S737) levels (H), and an increase in the ratio of p-CFTR (S737) to CFTR (I) in the PTS noise group compared to controls. Data are presented as means + SD, n = 3