MAST2 Antibody - #AF0571
(2)
| 产品: | MAST2 抗体 |
| 货号: | AF0571 |
| 描述: | Rabbit polyclonal antibody to MAST2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 196kDa; 196kD(Calculated). |
| 蛋白号: | Q6P0Q8 |
| RRID: | AB_2834288 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse
预测:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MAST2 Antibody detects endogenous levels of total MAST2.
RRID:
AB_2834288
引用格式: Affinity Biosciences Cat# AF0571, RRID:AB_2834288.
引用格式: Affinity Biosciences Cat# AF0571, RRID:AB_2834288.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
EC 2.7.11.1 3; FLJ39200; KIAA0807; MAST 2; MAST 205; Mast2; MAST2_HUMAN; Microtubule associated serine/threonine kinase 2; Microtubule associated testis specific serine/threonine protein kinase; Microtubule-associated serine/threonine-protein kinase 2; MTSSK; OTTHUMP00000009697 2; OTTHUMP00000046616; RP4 533D7.1; RP4-533D7.1 2;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
表达:
描述:
MAST2 appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappaB activation. 2 isoforms of the human protein are produced by alternative splicing.
序列:
- Q6P0Q8 MAST2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRSRCRDRPQPPPPDRREDGVQRAAELSQSLPPRRRAPPGRQRLEERTGPAGPEGKEQDVVTGVSPLLFRKLSNPDIFSSTGKVKLQRQLSQDDCKLWRGNLASSLSGKQLLPLSSSVHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESVPDEEGRQSPAMRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDSVLPLADGALSFIHHQVIEMARDCLDKSRSGLITSQYFYELQDNLEKLLQDAHERSESSEVAFVMQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSCDSPDTPETDDSIEGHGASLPSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDEALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHMDSEDEEEVSEDGCLEIRQFSSCSPRFNKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGLKGRDRSWVIGSPEILRKRLSVSESSHTESDSSPPMTVRRRCSGLLDAPRFPEGPEEASSTLRRQPQEGIWVLTPPSGEGVSGPVTEHSGEQRPKLDEEAVGRSSGSSPAMETRGRGTSQLAEGATAKAISDLAVRRARHRLLSGDSTEKRTARPVNKVIKSASATALSLLIPSEHHTCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALGSMRPPIIIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVAISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPDAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPPTASPQRSPSPLSGHVAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKHSLDLPHSELKKELPPREVSPLEVVGARSVLSGKGALPGKGVLQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
91
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q6P0Q8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T63 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| R163 | Methylation | Uniprot | |
| R169 | Methylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| Y461 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| K797 | Ubiquitination | Uniprot | |
| S806 | Phosphorylation | Uniprot | |
| Y812 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| Y853 | Phosphorylation | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| T867 | Phosphorylation | Uniprot | |
| T871 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| S883 | Phosphorylation | Uniprot | |
| K890 | Ubiquitination | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| S900 | Phosphorylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S931 | Phosphorylation | Uniprot | |
| S993 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| S996 | Phosphorylation | Uniprot | |
| R1004 | Methylation | Uniprot | |
| S1007 | Phosphorylation | Uniprot | |
| S1032 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | Uniprot | |
| S1180 | Phosphorylation | Uniprot | |
| K1183 | Ubiquitination | Uniprot | |
| S1195 | Phosphorylation | Uniprot | |
| K1197 | Ubiquitination | Uniprot | |
| K1203 | Acetylation | Uniprot | |
| K1207 | Acetylation | Uniprot | |
| K1209 | Acetylation | Uniprot | |
| K1237 | Ubiquitination | Uniprot | |
| S1240 | Phosphorylation | Uniprot | |
| S1245 | Phosphorylation | Uniprot | |
| S1247 | Phosphorylation | Uniprot | |
| S1250 | Phosphorylation | Uniprot | |
| S1256 | Phosphorylation | Uniprot | |
| S1264 | Phosphorylation | Uniprot | |
| S1275 | Phosphorylation | Uniprot | |
| S1337 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S1340 | Phosphorylation | Uniprot | |
| S1344 | Phosphorylation | Uniprot | |
| T1349 | Phosphorylation | Uniprot | |
| S1351 | Phosphorylation | Uniprot | |
| S1364 | Phosphorylation | Uniprot | |
| S1381 | Phosphorylation | Uniprot | |
| S1393 | Phosphorylation | Uniprot | |
| S1399 | Phosphorylation | Uniprot | |
| K1403 | Methylation | Uniprot | |
| S1407 | Phosphorylation | Uniprot | |
| S1425 | Phosphorylation | Uniprot | |
| S1429 | Phosphorylation | Uniprot | |
| S1435 | Phosphorylation | Uniprot | |
| S1447 | Phosphorylation | Uniprot | |
| S1503 | Phosphorylation | Uniprot | |
| S1504 | Phosphorylation | Uniprot | |
| T1508 | Phosphorylation | Uniprot | |
| S1541 | Phosphorylation | Uniprot | |
| S1554 | Phosphorylation | Uniprot | |
| T1564 | Phosphorylation | Uniprot | |
| T1567 | Phosphorylation | Uniprot | |
| S1585 | Phosphorylation | Uniprot | |
| S1631 | Phosphorylation | Uniprot | |
| S1642 | Phosphorylation | Uniprot | |
| K1659 | Methylation | Uniprot | |
| S1660 | Phosphorylation | Uniprot | |
| S1669 | Phosphorylation | Uniprot | |
| S1698 | Phosphorylation | Uniprot | |
| S1717 | Phosphorylation | Uniprot | |
| S1768 | Phosphorylation | Uniprot | |
| S1771 | Phosphorylation | Uniprot | |
| T1798 | Phosphorylation | Uniprot |
研究背景
功能:
Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation (By similarity).
翻译修饰:
Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination (By similarity).
细胞定位:
Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Recruited to the sub-membranous area on interaction with CDHR2.
组织特异性:
Abundant in the testis.
亚基结构:
Interacts with CDHR2.
蛋白家族:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
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