Phospho-NFAT2 (Ser294) Antibody - #AF8012

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产品: 磷酸化 NFAT2 (Ser294) 抗体
货号: AF8012
描述: Rabbit polyclonal antibody to Phospho-NFAT2 (Ser294)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Rabbit
蛋白号: O95644
RRID: AB_2840075

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-NFAT2 (Ser294) Antibody detects endogenous levels of NFAT2 only when phosphorylated at Ser294.
RRID:
AB_2840075
引用格式: Affinity Biosciences Cat# AF8012, RRID:AB_2840075.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cytoplasmic 1; MGC138448; NF ATc; NF ATc1; NF-ATc; NF-ATc1; NF-ATc1.2; NFAC1_HUMAN; NFAT 2; NFAT transcription complex cytosolic component; NFATC 1; NFATc; NFATc1; Nuclear factor of activated T cells cytoplasmic 1; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1; Nuclear factor of activated T cells cytosolic component 1; nuclear factor of activated T-cells 'c'; Nuclear factor of activated T-cells;

抗原和靶标

免疫原:

A synthesized peptide derived from human NFAT2 around the phosphorylation site of Ser294.

基因/基因ID:
NFATC1(4772)

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis. Cellular & Molecular Biology Letters, 2023 (PubMed: 37501148) [IF=9.2]

Application: WB    Species: Rat    Sample:

Fig. 5 The activity of NFATc1 induced by DYRK1A in early puberty. A Western blot show the expression of NFATc1 related phosphokinases and their phosphorylation in VS of PND-25 and PND-35. B Western blot show the phosphorylation of NFATc1 affected by DYRK1A inhibitor. C IF show the localization of NFATc1 and phosphorylated NFATc1 in VS of PND-25, PND25 with DYRK1A inhibitor and PND-35 (magnification ×200). The SG specimens from two individuals were used for western blot. The SG specimens from three individuals were used for immunofluorescence

Application: IF/ICC    Species: Rat    Sample:

Fig. 5 The activity of NFATc1 induced by DYRK1A in early puberty. A Western blot show the expression of NFATc1 related phosphokinases and their phosphorylation in VS of PND-25 and PND-35. B Western blot show the phosphorylation of NFATc1 affected by DYRK1A inhibitor. C IF show the localization of NFATc1 and phosphorylated NFATc1 in VS of PND-25, PND25 with DYRK1A inhibitor and PND-35 (magnification ×200). The SG specimens from two individuals were used for western blot. The SG specimens from three individuals were used for immunofluorescence
2). Cerium Oxide Nanoparticles Regulate Osteoclast Differentiation Bidirectionally by Modulating the Cellular Production of Reactive Oxygen Species. International Journal of Nanomedicine, 2020 (PubMed: 32922006) [IF=6.6]

Application: WB    Species: mouse    Sample: BMMs

Figure 6 CeO2NPs modulate osteoclast-specific gene expression via up- or downregulating the MAPK pathway, NF-κB pathway, and Nfatc1 signaling in a concentrationdependent manner. Notes: (A–F) qPCR analysis of expression of the osteoclast-specific genes Acp5, Ctsk, Dcstamp, Traf6, C-fos, and Calcr relative to Beta-actin in BMMs that were stimulated with RANKL for 4 days in the presence of various concentrations of CeO2NPs (n=3 per group). (G and H) Western blot analysis of the MAPK and NF-κB pathways. BMMs were pretreated with different concentrations of CeO2NPs for 24 h before stimulation with RANKL for 20 min. (I) Western blot of the translocation of dephosphorylated Nfatc1 into the nuclei of BMMs. BMMs were pretreated with different concentrations of CeO2NPs for 24 h before stimulation with RANKL for 40 min. All bar graphs are presented as the mean ± SD. **Indicates p<0.01 compared with the vehicle group (0 mg/L, RANKL+).

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