产品: STEA3 抗体
货号: AF0652
描述: Rabbit polyclonal antibody to STEA3
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 54kDa; 55kD(Calculated).
蛋白号: Q658P3
RRID: AB_2834284

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
STEA3 Antibody detects endogenous levels of total STEA3.
RRID:
AB_2834284
引用格式: Affinity Biosciences Cat# AF0652, RRID:AB_2834284.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1010001D01Rik; Dudlin 2; Dudlin2; Dudulin 2; Dudulin-2; Dudulin2; hpHyde; hTSAP6; Metalloreductase STEAP3; MGC93147; pHyde; Six transmembrane epithelial antigen of prostate 3; Six transmembrane prostate protein 3; Six-transmembrane epithelial antigen of prostate 3; STEA 3; STEA3_HUMAN; STEAP 3; STEAP family member 3; STEAP family member 3 metalloreductase; STEAP3; STMP 3; STMP3; TSAP 6; TSAP6; Tumor suppressor activated pathway protein 6; Tumor suppressor pHyde; Tumor suppressor-activated pathway protein 6;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q658P3 STEA3_HUMAN:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

描述:
STEAP3 a multi-pass membrane protein induced directly by p53. Plays an important role in facilitating exosome secretion. Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. Highly expressed in hematopoietic tissues where it localizes to the specialized endosome. An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor.
序列:
MPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRNPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWEVEAMPLRLLPAWKVPTLLALGLFVCFYAYNFVRDVLQPYVQESQNKFFKLPVSVVNTTLPCVAYVLLSLVYLPGVLAAALQLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFCAALHALYSFCLPLRRAHRYDLVNLAVKQVLANKSHLWVEEEVWRMEIYLSLGVLALGTLSLLAVTSLPSIANSLNWREFSFVQSSLGFVALVLSTLHTLTYGWTRAFEESRYKFYLPPTFTLTLLVPCVVILAKALFLLPCISRRLARIRRGWERESTIKFTLPTDHALAEKTSHV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Dog
90
Chicken
90
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.

翻译修饰:

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.

Glycosylated.

细胞定位:

Endosome membrane>Multi-pass membrane protein.
Note: Localizes to vesicular-like structures at the plasma membrane and around the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

亚基结构:

Homodimer. Interacts with BNIP3L, MYT1, RHBDL4/RHBDD1 and TCTP.

蛋白家族:

Belongs to the STEAP family.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

文献引用

1). Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte. JOURNAL OF NANOBIOTECHNOLOGY, 2025 [IF=12.6]

Application: WB    Species: Mouse    Sample: GC-2 cells

Fig. 3 PS-NPs initiate ferroptosis by triggering iron overload and ROS overproduction in GC-2 cells. (A) Various concentrations of PS-NPs (50 µg/mL, 100 µg/mL, 200 µg/mL) in different treatment times (6–24 h) reduce cell viability in GC-2 cells. (B and C) Representative images and quantification of fluorescence intensity for lipid peroxidation levels labelled with C11 BODIPY in GC-2 cells post-exposed with PS-NPs. Statistical analysis presented the ratio of MFI of green to red. (D-F) The levels of MDA, GSH, and total Fe were detected in GC-2 cells after PS-NPs treatment for 12 h. (G and H) Representative images and quantification of fluorescence intensity showing intracellular chelatable iron in GC-2 cells post-exposure to PS-NPs labelled with FerroOrange (red). (I and J) DCFH-DA staining was performed to detect ROS levels of GC-2 cells after PS-NPs exposure for 12 h using fluorescent microscopy. (K) Flow cytometry analysis of and ROS levels of GC-2 cells after PS-NPs exposure for 12 h using Fluorescence-activated cell sorting (FACS). (L) qRT-PCR analysis of ferroptosis-related gene expression in GC-2 cells treated with or without PS-NPs. (M and N) Ferroptosis-related proteins in GC-2 cells stimulated by PS-NPs were determined by western blotting. And semi-quantification of protein expression levels normalized to β-actin. (O) Representative TEM images depicting mitochondria in GC-2 cells in response to exposure to PS-NPs. The reduction of mitochondrial cristae is denoted by yellow arrows, while red arrows signify mitochondrial membrane rupture. (P and Q) Representative illustration and quantification of fluorescence intensity depicting mitochondria in GC-2 cells after treatment with PS-NPs using Mito-Tracker Green. (R and S) Illustration and quantification data of mitochondrial membrane potential in GC-2 cells in response to treatment of PS-NPs stained with TMRE

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.