产品: NDF2 抗体
货号: AF0635
描述: Rabbit polyclonal antibody to NDF2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Dog
分子量: 48kDa; 41kD(Calculated).
蛋白号: Q15784
RRID: AB_2834265

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
克隆:
Polyclonal
特异性:
NDF2 Antibody detects endogenous levels of total NDF2.
RRID:
AB_2834265
引用格式: Affinity Biosciences Cat# AF0635, RRID:AB_2834265.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLHa1; class A basic helix loop helix protein 1; Class A basic helix-loop-helix protein 1; NDF2_HUMAN; NDR2; NDRF; neuroD related factor; NeuroD-related factor; NeuroD2; neurogenic basic helix loop helix protein; neurogenic differentiation 2; Neurogenic differentiation factor 2; neuronal differentiation 2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
NEUROD2 Appears to mediate neuronal differentiation. Efficient DNA binding requires dimerization with another bHLH protein.
序列:
MLTRLFSEPGLLSDVPKFASWGDGEDDEPRSDKGDAPPPPPPAPGPGAPGPARAAKPVPLRGEEGTEATLAEVKEEGELGGEEEEEEEEEEGLDEAEGERPKKRGPKKRKMTKARLERSKLRRQKANARERNRMHDLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKRPDLVSYVQTLCKGLSQPTTNLVAGCLQLNSRNFLTEQGADGAGRFHGSGGPFAMHPYPYPCSRLAGAQCQAAGGLGGGAAHALRTHGYCAAYETLYAAAGGGGASPDYNSSEYEGPLSPPLCLNGNFSLKQDSSPDHEKSYHYSMHYSALPGSRPTGHGLVFGSSAVRGGVHSENLLSYDMHLHHDRGPMYEELNAFFHN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15784 作为底物

Site PTM Type Enzyme
S7 Phosphorylation
S20 Phosphorylation
K156 Ubiquitination
Y169 Phosphorylation
S212 Phosphorylation

研究背景

功能:

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with TCF3, TCF4 and TCF12. Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein (By similarity).

蛋白家族:

The C-terminal region is necessary for depolarization-induced and calcium-dependent transcription activation.

文献引用

1). Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s Disease. Frontiers in Pharmacology (PubMed: 35694256) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample: brain tissue

FIGURE 7 Experimental validation of HER2 and NDF2. (A) Immunofluorescence expression of HER2 (8.5 months). (B) Immunofluorescence expression of NDF2 (8.5 months). (C) Western blot results of NDF2 and HER2 (17 months). (D,E) Statistical analysis of immunofluorescence and western blot.

Application: WB    Species: Mouse    Sample: brain tissue

FIGURE 7 Experimental validation of HER2 and NDF2. (A) Immunofluorescence expression of HER2 (8.5 months). (B) Immunofluorescence expression of NDF2 (8.5 months). (C) Western blot results of NDF2 and HER2 (17 months). (D,E) Statistical analysis of immunofluorescence and western blot.

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