EGR1 Antibody - #AF0589

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产品: EGR1 抗体
货号: AF0589
描述: Rabbit polyclonal antibody to EGR1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Monkey
蛋白号: P18146
RRID: AB_2834263

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Monkey
克隆:
Polyclonal
特异性:
EGR1 Antibody detects endogenous levels of total EGR1.
RRID:
AB_2834263
引用格式: Affinity Biosciences Cat# AF0589, RRID:AB_2834263.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AT225;Early growth response 1;Early growth response protein 1;EGR 1;EGR-1;EGR1;EGR1_HUMAN;G0S30;KROX 24;KROX24;Nerve growth factor-induced clone A;Nerve growth factor-induced protein A;NGFI-A;NGFIA;TIS8;Transcription factor ETR103;Transcription factor Zif268;ZIF 268;ZIF268;Zinc finger protein 225;Zinc finger protein Krox-24;ZNF225;

抗原和靶标

免疫原:

A synthesized peptide derived from human EGR1, corresponding to a region within the internal amino acids.

基因/基因ID:
EGR1(1958)
描述:
NAB2 acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

文献引用

1). Pyrvinium pamoate can overcome artemisinin’s resistance in anaplastic thyroid cancer. BMC Complementary Medicine and Therapies, 2021 (PubMed: 34049534) [IF=3.9]

Application: WB    Species: Human    Sample: CAL-62 cells

Fig. 1 Effects of artemisinin (ART) on anaplastic thyroid cancer. A CCK-8 proliferation assay after ART treatment for 24 and 48 h. B Flow cytometry of cells treated with or without 100 μM ART for 48 h. C Western blotting of cyclin D1, ANGPTL2, and EGR1 after treatment with 100 μM ART for 48 h. *, statistical significance (p < 0.05); NS, not significant (p > 0.05); error bars, mean ± standard deviation; ratio, the ratio of integrated density (treatment and untreated cells). Gels/blots are cropped for a clear presentation of results. Protein samples derived from the same experiment and gels/blots were processed in parallel. N, groups that did not receive ART treatment. ART, groups that received 100 μM ART treatment. Red, G1/G0; yellow, G2/M; slash, S

Application: WB    Species: human    Sample: CAL-62 cells;BHT-101 cells

Fig. 1| Effects of artemisinin (ART) on anaplastic thyroid cancer.C Western blotting of cyclin D1, ANGPTL2, and EGR1 after treatment with 100 μM ART for 48 h. *, statistical significance (p < 0.05); NS, not significant (p > 0.05); error bars, mean ± standard deviation; ratio, the ratio of integrated density(treatment and untreated cells). Gels/blots are cropped for a clear presentation of results. Protein samples derived from the same experiment and gels/blots were processed in parallel. N, groups that did not receive ART treatment. ART, groups that received 100 μM ART treatment. Red, G1/G0;yellow, G2/M; slash, S
2). The Effect of EGR1 on the Proliferation of Dermal Papilla Cells. Genes, 2022 (PubMed: 35886025) [IF=3.5]
3). Ramelteon improves blood-brain barrier of focal cerebral ischemia rats to prevent post-stroke depression via upregulating occludin. Behavioural brain research, 2023 (PubMed: 37146721) [IF=2.6]
4). KMT2D Regulates the NCOA6/THRB Signal Axis through Epigenetic Modification to Promote the Migration and Invasion of Papillary Thyroid Cancer. Frontiers in Bioscience - Elite, 2023 (PubMed: 36722273)

Application: WB    Species: Human    Sample: thyroid papillary carcinoma cells

Fig. 3. The mechanism of KMT2D on papillary thyroid carcinoma cells. (a) ChIP assay. (b) qRT-PCR analysis. (c) Western blotting analysis. A, si-NC; B, si-KMT2D (1); C, si- KMT2D (1).

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