EPHA7 Antibody - #AF0627

Fig. 5 Adipose RBM3 mediates the action of S100A8+ immune cells in sympathetic innervation. a Schematic diagram of experimental processes. This diagram was created with BioRender.com. b Representative images of TH staining in the BAT of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells. Scale bar, 100 µm. c Representative immunoblots of UCP1, TH, p-TH, TUBB3 and RBM3 in the BAT of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells (n = 3). d Relative mRNA levels of Rbm3, Th Ucp1, Ppargc1α, p16, and p21 in the BAT of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells (n = 5). e Oxygen consumption of mice injected with AAV-RBM3 or AAV-NC and transferred with S100A8+ immune cells or S100A8- immune cells (n = 6–7). f Core body temperature of mice injected with AAV-RBM3 or AAV-NC were transferred with S100A8+ immune cells and subjected to cold challenge (n = 5). g Venn diagram showing overlapped genes which are RBM3-bounded and differentially expressed upon Rbm3 knockdown. h KEGG analysis of the 909 overlapped genes shown in (g). i Heatmap of axon guidance-related gene expressions based on RNA-seq data of Rbm3 knockdown differentiated brown adipocytes. j Relative mRNA levels of Rbm3 and axon guidance-related genes Nrp1 and Epha7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC (n = 3). k Representative immunoblots of NRP1 and EPHA7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC (n = 4). l RNA immunoprecipitation (RIP) assay assessing RBM3 binding on 3´UTR of Nrp1 and Epha7 in differentiated brown adipocytes (n = 4). m Relative mRNA level of Nrp1 and Epha7 in si-Rbm3 or si-NC transfected brown adipocytes upon transcriptional inhibition with actinomycin D at indicated time (n = 3). n Representative images of TUBB3 staining in PC12 cells cocultured with differentiated brown adipocytes overexpressed with RBM3 and transfected with si-Nrp1 or si-Epha7 (n = 4). Scale bar, 100 µm. Data shown are representative of three independent experiments with similar results. n indicates the number of biologically independent samples examined. Data are shown as the mean ± SEM. Statistical differences were supposed to be significant when P