产品: | EPHA7 抗体 |
货号: | AF0627 |
描述: | Rabbit polyclonal antibody to EPHA7 |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 112kDa; 112kD(Calculated). |
蛋白号: | Q15375 |
RRID: | AB_2834259 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0627, RRID:AB_2834259.
展开/折叠
Cek 11; Developmental kinase 1; EBK; EHK-3; EHK3; EK11; Embryonic brain kinase; EPH homology kinase 3; EPH-like kinase 11; Epha7; EPHA7_HUMAN; Ephrin receptor Eph A7; Ephrin type A receptor 7; Ephrin type-A receptor 7; hEK11; MDK 1; Receptor protein tyrosine kinase HEK 11; Tyrosine protein kinase receptor EHK 3;
抗原和靶标
- Q15375 EPHA7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVFQTRYPSWIILCYIWLLRFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPNGWEEISGLDENYTPIRTYQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRENLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIENLAIFPDTVTGSEFSSLVEVRGTCVSSAEEEAENAPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCGRGFYKSSSQDLQCSRCPTHSFSDKEGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLEWSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNGVSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVELSWQEPEHPNGVITEYEIKYYEKDQRERTYSTVKTKSTSASINNLKPGTVYVFQIRAFTAAGYGNYSPRLDVATLEEATGKMFEATAVSSEQNPVIIIAVVAVAGTIILVFMVFGFIIGRRHCGYSKADQEGDEELYFHFKFPGTKTYIDPETYEDPNRAVHQFAKELDASCIKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGVVTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNLVCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDCWQKERAERPKFEQIVGILDKMIRNPNSLKTPLGTCSRPISPLLDQNTPDFTTFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIEDVMSLGITLVGHQKKIMSSIQTMRAQMLHLHGTGIQV
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15375 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T87 | Phosphorylation | Uniprot | |
S91 | Phosphorylation | Uniprot | |
Y201 | Phosphorylation | Uniprot | |
Y202 | Phosphorylation | Uniprot | |
S307 | Phosphorylation | Uniprot | |
S308 | Phosphorylation | Uniprot | |
S501 | Phosphorylation | Uniprot | |
Y597 | Phosphorylation | Uniprot | |
K601 | Ubiquitination | Uniprot | |
K606 | Ubiquitination | Uniprot | |
T607 | Phosphorylation | Uniprot | |
Y608 | Phosphorylation | Uniprot | |
T613 | Phosphorylation | Uniprot | |
Y614 | Phosphorylation | Uniprot | |
K634 | Ubiquitination | Uniprot | |
K657 | Ubiquitination | Uniprot | |
T666 | Phosphorylation | Uniprot | |
K668 | Ubiquitination | Uniprot | |
Y671 | Phosphorylation | Uniprot | |
Y748 | Phosphorylation | Uniprot | |
Y754 | Phosphorylation | Uniprot | |
S768 | Phosphorylation | Uniprot | |
S775 | Phosphorylation | Uniprot | |
S780 | Phosphorylation | Uniprot | |
Y791 | Phosphorylation | Uniprot | |
T792 | Phosphorylation | Uniprot | |
T794 | Phosphorylation | Uniprot | |
K797 | Ubiquitination | Uniprot | |
Y810 | Phosphorylation | Uniprot | |
K892 | Ubiquitination | Uniprot |
研究背景
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.
Phosphorylated.
Cell membrane>Single-pass type I membrane protein.
Widely expressed.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts (via PDZ-binding motif) with GRIP1 and PICK1 (via PDZ domain) (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
研究领域
· Organismal Systems > Development > Axon guidance. (View pathway)
文献引用
Application: WB Species: Mouse Sample:
限制条款
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