产品: ANKRD1 抗体
货号: AF0677
描述: Rabbit polyclonal antibody to ANKRD1
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 36kDa; 36kD(Calculated).
蛋白号: Q15327
RRID: AB_2834258

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
ANKRD1 Antibody detects endogenous levels of total ANKRD1.
RRID:
AB_2834258
引用格式: Affinity Biosciences Cat# AF0677, RRID:AB_2834258.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ALRP; ANKR1_HUMAN; ANKRD 1; ANKRD1; Ankyrin repeat domain 1 (cardiac muscle); Ankyrin repeat domain containing protein 1; Ankyrin repeat domain-containing protein 1; bA320F15.2; C 193; C193; Cardiac ankyrin repeat protein; CARP; CVARP; Cytokine inducible nuclear protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; HA1A2; Liver ankyrin repeat domain 1; MCARP; OTTHUMP00000020084;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q15327 ANKR1_HUMAN:

Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells.

描述:
The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor.
序列:
MMVLKVEELVTGKKNGNGEAGEFLPEDFRDGEYEAAVTLEKQEDLKTLLAHPVTLGEQQWKSEKQREAELKKKKLEQRSKLENLEDLEIIIQLKKRKKYRKTKVPVVKEPEPEIITEPVDVPTFLKAALENKLPVVEKFLSDKNNPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWASRGGNLDVLKLLLNKGAKISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMYGADLNIKNCAGKTPMDLVLHWQNGTKAIFDSLRENSYKTSRIATF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15327 作为底物

Site PTM Type Enzyme
Y274 Phosphorylation
S310 Phosphorylation
Y311 Phosphorylation

研究背景

功能:

May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells.

亚基结构:

Interacts with YBX1 (By similarity). Interacts with TTN/titin.

文献引用

1). Storax Attenuates Cardiac Fibrosis following Acute Myocardial Infarction in Rats via Suppression of AT1R–Ankrd1–P53 Signaling Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (PubMed: 36361958) [IF=5.6]

Application: WB    Species: Rat    Sample: cardiac tissue

Figure 8 Storax inhibits the AT1R–Ankrd1–P53 pathway in AMI rats against cardiomyocyte apoptosis. (A) WB bands showing the protein expression levels of AT1R, Ankrd1, P-p53 (ser15), P53 and Mdm2 in cardiac tissue. (B–E) Relative protein for AT1R, Ankrd1, P-p53 (ser15) and Mdm2 were quantified by densitometry based on immunoblot images. Results are presented as mean ± SD (n = 3). One-way ANOVA follow by Bonferroni’s post hoc test: # p < 0.05, ## p < 0.01 vs. vehicle group.

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