产品: GYS1 抗体
货号: BF0469
描述: Mouse monoclonal antibody to GYS1
应用: WB ELISA FACS
反应: Human, Mouse
分子量: 85kDa; 84kD(Calculated).
蛋白号: P13807
RRID: AB_2833883

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 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Monoclonal [AFB1859]
特异性:
GYS1 antibody detects endogenous levels of total GYS1.
RRID:
AB_2833883
引用格式: Affinity Biosciences Cat# BF0469, RRID:AB_2833883.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Glycogen [starch] synthase; Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; GYS; Gys1; GYS1_HUMAN; muscle;

抗原和靶标

免疫原:

Purified recombinant fragment of human GYS1 expressed in E. Coli.

Uniprot:
基因/基因ID:
描述:
GYS1 muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.
序列:
MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVYFGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTWFLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEFSNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN

翻译修饰 - P13807 作为底物

Site PTM Type Enzyme
Phosphorylation
T6 Phosphorylation
S8 Phosphorylation
S10 Phosphorylation
S11 Phosphorylation
Y44 Phosphorylation
K52 Ubiquitination
T75 Phosphorylation
K88 Acetylation
K88 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K100 Acetylation
Y102 Phosphorylation
K231 Ubiquitination
K271 Ubiquitination
K273 Ubiquitination
K285 Ubiquitination
K286 Ubiquitination
K301 Ubiquitination
K381 Ubiquitination
K387 Ubiquitination
K397 Ubiquitination
K403 Ubiquitination
Y405 Phosphorylation
S412 Phosphorylation
K418 Ubiquitination
K422 Ubiquitination
T426 Phosphorylation
K429 Ubiquitination
S484 Phosphorylation
T485 Phosphorylation
S486 Phosphorylation
K599 Ubiquitination
Y604 Phosphorylation
K615 Ubiquitination
Y636 Phosphorylation
S641 Phosphorylation Q96RG2 (PASK) , P49841 (GSK3B) , P49840 (GSK3A) , Q92630 (DYRK2)
S645 Phosphorylation Q15759 (MAPK11) , P49841 (GSK3B)
S647 Phosphorylation
S649 Phosphorylation
S652 Phosphorylation
S653 Phosphorylation P49841 (GSK3B) , Q15759 (MAPK11)
S657 Phosphorylation
Y675 Phosphorylation
K683 Ubiquitination
S698 Phosphorylation
T700 Phosphorylation
S710 Phosphorylation
T713 Phosphorylation
T715 Phosphorylation
S716 Phosphorylation
S717 Phosphorylation
S718 Phosphorylation
T721 Phosphorylation Q15759 (MAPK11)
S723 Phosphorylation
S727 Phosphorylation Q15759 (MAPK11)
T729 Phosphorylation
S730 Phosphorylation
S731 Phosphorylation

研究背景

功能:

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

翻译修饰:

Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.

亚基结构:

Interacts with GYG1.

蛋白家族:

Belongs to the glycosyltransferase 3 family.

研究领域

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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