产品: JAK3 抗体
货号: BF0256
描述: Mouse monoclonal antibody to JAK3
反应: Human, Mouse
分子量: 125kDa; 125kD(Calculated).
蛋白号: P52333
RRID: AB_2833738


   规格 价格 库存
 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

货期: 当天发货



ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

Monoclonal [AFB1714]
JAK3 antibody detects endogenous levels of total JAK3.
引用格式: Affinity Biosciences Cat# BF0256, RRID:AB_2833738.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


EC; JAK 3; JAK L; JAK-3; Jak3; JAK3 HUMAN; JAK3_HUMAN; JAKL; Janus kinase 3 (a protein tyrosine kinase, leukocyte); Janus kinase 3; Janus Kinase3; L JAK; L-JAK; Leukocyte janus kinase; LJAK; Protein tyrosine kinase leukocyte; Tyrosine protein kinase JAK3; Tyrosine-protein kinase JAK3;



Purified recombinant fragment of human JAK3 expressed in E. Coli.

P52333 JAK3_HUMAN:

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).

翻译修饰 - P52333 作为底物

Site PTM Type Enzyme
S5 Phosphorylation
T8 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
S20 Phosphorylation
K122 Ubiquitination
K157 Ubiquitination
K186 Ubiquitination
K237 Ubiquitination
K361 Ubiquitination
K388 Ubiquitination
K390 Ubiquitination
T391 Phosphorylation
S398 Phosphorylation
K482 Ubiquitination
S498 Phosphorylation
Y506 Phosphorylation
K534 Ubiquitination
Y536 Phosphorylation
S568 Phosphorylation
K624 Ubiquitination
K652 Ubiquitination
S663 Phosphorylation
K734 Ubiquitination
K748 Acetylation
S782 Phosphorylation
Y785 Phosphorylation P52333 (JAK3)
K823 Ubiquitination
K830 Ubiquitination
K855 Ubiquitination
K876 Ubiquitination
S880 Phosphorylation
Y886 Phosphorylation
Y904 Phosphorylation P52333 (JAK3)
Y929 Phosphorylation
S931 Phosphorylation
K935 Ubiquitination
Y939 Phosphorylation P52333 (JAK3)
S959 Phosphorylation
K972 Ubiquitination
K978 Ubiquitination
Y980 Phosphorylation P52333 (JAK3)
Y981 Phosphorylation P52333 (JAK3)
K1117 Ubiquitination
S1120 Phosphorylation

翻译修饰 - P52333 作为激酶

Substrate Site Source
O14939 (PLD2) Y415 Uniprot
O95644 (NFATC1) Y371 Uniprot
P23458 (JAK1) Y1034 Uniprot
P23458 (JAK1) Y1035 Uniprot
P29353-2 (SHC1) Y410 Uniprot
P29353-2 (SHC1) Y448 Uniprot
P42224 (STAT1) Y701 Uniprot
P42229 (STAT5A) Y694 Uniprot
P52333 (JAK3) Y785 Uniprot
P52333 (JAK3) Y904 Uniprot
P52333 (JAK3) Y939 Uniprot
P52333-1 (JAK3) Y980 Uniprot
P52333 (JAK3) Y981 Uniprot
Q15717 (ELAVL1) Y63 Uniprot
Q15717 (ELAVL1) Y68 Uniprot
Q15717 (ELAVL1) Y200 Uniprot
Q96LC7 (SIGLEC10) Y597 Uniprot
Q96LC7 (SIGLEC10) Y667 Uniprot
Q96LC7 (SIGLEC10) Y691 Uniprot



Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.


Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).


Endomembrane system>Peripheral membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.


Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.


Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.


· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)


1). Chen L et al. Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway. ONCOLOGY REPORTS 2018 Mar;39(3):1081-1089 (PubMed: 29328487) [IF=4.2]

Application: WB    Species: human    Sample: BxPC-3 and PANC‑1 cells

Figure 3.| The gemcitabine-erlotinib (E+G) combination group inhibits the activity of the JAK-STAT pathway, as well as the expression of downstream HIF‑1α, cyclin D1 and p53. (E) Phosphorylation levels of JAK1 (Tyr1022), JAK2 (Tyr221), JAK3(Tyr981) and STAT3 (Tyr701) in BxPC-3 and PANC‑1 cells were analyzed by western blotting, respectively.

2). Xu L et al. Scorpion venom polypeptide governs alveolar macrophage M1/M2 polarization to alleviate pulmonary fibrosis. Tissue and Cell 2022 Sep 17;79:101939 (PubMed: 36179453) [IF=2.6]


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