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  • 名 称:
    Ki67 抗体
  • 货 号:
    AF0198
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,IF/ICC,ELISA
  • 反 应:
    Human, Mouse, Rat
  • 预 测:
    Bovine(100%), Rabbit(100%), Dog(82%)
  • 蛋白号:
  • 分子量:
    358kDa
  • 浓 度:
    1mg/ml
  • 浏览相似产品>>

产品信息

Alternative Names:Expand▼

Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen KI67; Antigen Ki67; KI67_HUMAN; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Bovine(100%), Rabbit(100%), Dog(82%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Ki67 Antibody detects endogenous levels of Ki67.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human Ki67, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 358kDa.
Predicted Mol.Wt.: 359kDa.

Subcellular Location:

Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).

Tissue Specificity:

Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).

Description:

KI-67 a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Sequence:
        10         20         30         40         50
MWPTRRLVTI KRSGVDGPHF PLSLSTCLFG RGIECDIRIQ LPVVSKQHCK
60 70 80 90 100
IEIHEQEAIL HNFSSTNPTQ VNGSVIDEPV RLKHGDVITI IDRSFRYENE
110 120 130 140 150
SLQNGRKSTE FPRKIREQEP ARRVSRSSFS SDPDEKAQDS KAYSKITEGK
160 170 180 190 200
VSGNPQVHIK NVKEDSTADD SKDSVAQGTT NVHSSEHAGR NGRNAADPIS
210 220 230 240 250
GDFKEISSVK LVSRYGELKS VPTTQCLDNS KKNESPFWKL YESVKKELDV
260 270 280 290 300
KSQKENVLQY CRKSGLQTDY ATEKESADGL QGETQLLVSR KSRPKSGGSG
310 320 330 340 350
HAVAEPASPE QELDQNKGKG RDVESVQTPS KAVGASFPLY EPAKMKTPVQ
360 370 380 390 400
YSQQQNSPQK HKNKDLYTTG RRESVNLGKS EGFKAGDKTL TPRKLSTRNR
410 420 430 440 450
TPAKVEDAAD SATKPENLSS KTRGSIPTDV EVLPTETEIH NEPFLTLWLT
460 470 480 490 500
QVERKIQKDS LSKPEKLGTT AGQMCSGLPG LSSVDINNFG DSINESEGIP
510 520 530 540 550
LKRRRVSFGG HLRPELFDEN LPPNTPLKRG EAPTKRKSLV MHTPPVLKKI
560 570 580 590 600
IKEQPQPSGK QESGSEIHVE VKAQSLVISP PAPSPRKTPV ASDQRRRSCK
610 620 630 640 650
TAPASSSKSQ TEVPKRGGRK SGNLPSKRVS ISRSQHDILQ MICSKRRSGA
660 670 680 690 700
SEANLIVAKS WADVVKLGAK QTQTKVIKHG PQRSMNKRQR RPATPKKPVG
710 720 730 740 750
EVHSQFSTGH ANSPCTIIIG KAHTEKVHVP ARPYRVLNNF ISNQKMDFKE
760 770 780 790 800
DLSGIAEMFK TPVKEQPQLT STCHIAISNS ENLLGKQFQG TDSGEEPLLP
810 820 830 840 850
TSESFGGNVF FSAQNAAKQP SDKCSASPPL RRQCIRENGN VAKTPRNTYK
860 870 880 890 900
MTSLETKTSD TETEPSKTVS TANRSGRSTE FRNIQKLPVE SKSEETNTEI
910 920 930 940 950
VECILKRGQK ATLLQQRREG EMKEIERPFE TYKENIELKE NDEKMKAMKR
960 970 980 990 1000
SRTWGQKCAP MSDLTDLKSL PDTELMKDTA RGQNLLQTQD HAKAPKSEKG
1010 1020 1030 1040 1050
KITKMPCQSL QPEPINTPTH TKQQLKASLG KVGVKEELLA VGKFTRTSGE
1060 1070 1080 1090 1100
TTHTHREPAG DGKSIRTFKE SPKQILDPAA RVTGMKKWPR TPKEEAQSLE
1110 1120 1130 1140 1150
DLAGFKELFQ TPGPSEESMT DEKTTKIACK SPPPESVDTP TSTKQWPKRS
1160 1170 1180 1190 1200
LRKADVEEEF LALRKLTPSA GKAMLTPKPA GGDEKDIKAF MGTPVQKLDL
1210 1220 1230 1240 1250
AGTLPGSKRQ LQTPKEKAQA LEDLAGFKEL FQTPGHTEEL VAAGKTTKIP
1260 1270 1280 1290 1300
CDSPQSDPVD TPTSTKQRPK RSIRKADVEG ELLACRNLMP SAGKAMHTPK
1310 1320 1330 1340 1350
PSVGEEKDII IFVGTPVQKL DLTENLTGSK RRPQTPKEEA QALEDLTGFK
1360 1370 1380 1390 1400
ELFQTPGHTE EAVAAGKTTK MPCESSPPES ADTPTSTRRQ PKTPLEKRDV
1410 1420 1430 1440 1450
QKELSALKKL TQTSGETTHT DKVPGGEDKS INAFRETAKQ KLDPAASVTG
1460 1470 1480 1490 1500
SKRHPKTKEK AQPLEDLAGL KELFQTPVCT DKPTTHEKTT KIACRSQPDP
1510 1520 1530 1540 1550
VDTPTSSKPQ SKRSLRKVDV EEEFFALRKR TPSAGKAMHT PKPAVSGEKN
1560 1570 1580 1590 1600
IYAFMGTPVQ KLDLTENLTG SKRRLQTPKE KAQALEDLAG FKELFQTRGH
1610 1620 1630 1640 1650
TEESMTNDKT AKVACKSSQP DPDKNPASSK RRLKTSLGKV GVKEELLAVG
1660 1670 1680 1690 1700
KLTQTSGETT HTHTEPTGDG KSMKAFMESP KQILDSAASL TGSKRQLRTP
1710 1720 1730 1740 1750
KGKSEVPEDL AGFIELFQTP SHTKESMTNE KTTKVSYRAS QPDLVDTPTS
1760 1770 1780 1790 1800
SKPQPKRSLR KADTEEEFLA FRKQTPSAGK AMHTPKPAVG EEKDINTFLG
1810 1820 1830 1840 1850
TPVQKLDQPG NLPGSNRRLQ TRKEKAQALE ELTGFRELFQ TPCTDNPTTD
1860 1870 1880 1890 1900
EKTTKKILCK SPQSDPADTP TNTKQRPKRS LKKADVEEEF LAFRKLTPSA
1910 1920 1930 1940 1950
GKAMHTPKAA VGEEKDINTF VGTPVEKLDL LGNLPGSKRR PQTPKEKAKA
1960 1970 1980 1990 2000
LEDLAGFKEL FQTPGHTEES MTDDKITEVS CKSPQPDPVK TPTSSKQRLK
2010 2020 2030 2040 2050
ISLGKVGVKE EVLPVGKLTQ TSGKTTQTHR ETAGDGKSIK AFKESAKQML
2060 2070 2080 2090 2100
DPANYGTGME RWPRTPKEEA QSLEDLAGFK ELFQTPDHTE ESTTDDKTTK
2110 2120 2130 2140 2150
IACKSPPPES MDTPTSTRRR PKTPLGKRDI VEELSALKQL TQTTHTDKVP
2160 2170 2180 2190 2200
GDEDKGINVF RETAKQKLDP AASVTGSKRQ PRTPKGKAQP LEDLAGLKEL
2210 2220 2230 2240 2250
FQTPICTDKP TTHEKTTKIA CRSPQPDPVG TPTIFKPQSK RSLRKADVEE
2260 2270 2280 2290 2300
ESLALRKRTP SVGKAMDTPK PAGGDEKDMK AFMGTPVQKL DLPGNLPGSK
2310 2320 2330 2340 2350
RWPQTPKEKA QALEDLAGFK ELFQTPGTDK PTTDEKTTKI ACKSPQPDPV
2360 2370 2380 2390 2400
DTPASTKQRP KRNLRKADVE EEFLALRKRT PSAGKAMDTP KPAVSDEKNI
2410 2420 2430 2440 2450
NTFVETPVQK LDLLGNLPGS KRQPQTPKEK AEALEDLVGF KELFQTPGHT
2460 2470 2480 2490 2500
EESMTDDKIT EVSCKSPQPE SFKTSRSSKQ RLKIPLVKVD MKEEPLAVSK
2510 2520 2530 2540 2550
LTRTSGETTQ THTEPTGDSK SIKAFKESPK QILDPAASVT GSRRQLRTRK
2560 2570 2580 2590 2600
EKARALEDLV DFKELFSAPG HTEESMTIDK NTKIPCKSPP PELTDTATST
2610 2620 2630 2640 2650
KRCPKTRPRK EVKEELSAVE RLTQTSGQST HTHKEPASGD EGIKVLKQRA
2660 2670 2680 2690 2700
KKKPNPVEEE PSRRRPRAPK EKAQPLEDLA GFTELSETSG HTQESLTAGK
2710 2720 2730 2740 2750
ATKIPCESPP LEVVDTTAST KRHLRTRVQK VQVKEEPSAV KFTQTSGETT
2760 2770 2780 2790 2800
DADKEPAGED KGIKALKESA KQTPAPAASV TGSRRRPRAP RESAQAIEDL
2810 2820 2830 2840 2850
AGFKDPAAGH TEESMTDDKT TKIPCKSSPE LEDTATSSKR RPRTRAQKVE
2860 2870 2880 2890 2900
VKEELLAVGK LTQTSGETTH TDKEPVGEGK GTKAFKQPAK RKLDAEDVIG
2910 2920 2930 2940 2950
SRRQPRAPKE KAQPLEDLAS FQELSQTPGH TEELANGAAD SFTSAPKQTP
2960 2970 2980 2990 3000
DSGKPLKISR RVLRAPKVEP VGDVVSTRDP VKSQSKSNTS LPPLPFKRGG
3010 3020 3030 3040 3050
GKDGSVTGTK RLRCMPAPEE IVEELPASKK QRVAPRARGK SSEPVVIMKR
3060 3070 3080 3090 3100
SLRTSAKRIE PAEELNSNDM KTNKEEHKLQ DSVPENKGIS LRSRRQNKTE
3110 3120 3130 3140 3150
AEQQITEVFV LAERIEINRN EKKPMKTSPE MDIQNPDDGA RKPIPRDKVT
3160 3170 3180 3190 3200
ENKRCLRSAR QNESSQPKVA EESGGQKSAK VLMQNQKGKG EAGNSDSMCL
3210 3220 3230 3240 3250
RSRKTKSQPA ASTLESKSVQ RVTRSVKRCA ENPKKAEDNV CVKKIRTRSH

RDSEDI

研究背景

Function:

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Post-translational Modifications:

Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (PubMed:19131338, PubMed:23178126).

Western blot analysis of extracts from various samples, using Ki67 Antibody. Lane 1: Mouse brain lysates; Lane 2: Rat spleen lysates; Lane 3: Rat spleen lysates treated with blocking peptide;
AF0198 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0198 at 1/200 staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0198 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0198 at 1/200 staining Human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0198 at 1/50 staining human lymphoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0198 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

Reference Citations:

1). Dong H et al. GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 2019 Jul 31;10(8):574 (PubMed: 31366904) [IF=5.959]

2). Lei D et al. Quercetin inhibited mesangial cell proliferation of early diabetic nephropathy through the Hippo pathway. Pharmacol Res 2019 Jun 17;146:104320 (PubMed: 31220559) [IF=5.574]

3). Liu H et al. Antimalarial Drug Pyrimethamine Plays a Dual Role in Antitumor Proliferation and Metastasis through Targeting DHFR and TP. Mol Cancer Ther 2019 Mar;18(3):541-555 (PubMed: 30642883) [IF=4.856]

4). Yang J et al. Oridonin inhibits oral cancer growth and PI3K/Akt signaling pathway. Biomed Pharmacother 2018 Apr;100:226-232 (PubMed: 29432993) [IF=3.743]

5). Chen Z et al. Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients. J Cancer 2019 Jan 30;10(5):1288-1296 (PubMed: 30854138) [IF=3.182]

6). Fang D et al. Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo. Int J Mol Med 2019 Aug 19 (PubMed: 31432159)

7). Chen G et al. CC chemokine ligand 18 correlates with malignant progression of prostate cancer. Biomed Res Int 2014;2014:230183 (PubMed: 25197632)

Application: IHC    Species:Not available;    Sample:Not available


8). Liu X et al. MicroRNA-370 inhibits the growth and metastasis of lung cancer by down-regulating epidermal growth factor receptor expression. Oncotarget 2017 Oct 4;8(50):88139-88151 (PubMed: 29152147)

Application: IHC    Species:human;    Sample:Not available

Figure 5: Induction of miR-370 over-expression inhibits the growth and angiogenesis of NSCLC xenograft tumors in vivo. (H) Histological and immunohistochemistry analysis.


9). Sun C et al. Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development. Oncotarget 2016 Feb 16;7(7):8341-59 (PubMed: 26840018)

Application: IHC    Species:Not available;    Sample:Not available

(D) Immunohistochemistry showed miR-326 decreased the proliferation index Ki67


10). Zhu Y et al. Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression. Tumour Biol 2016 Oct;37(10):14009-14023 (PubMed: 27492604)

Application: IHC    Species:mouse;    Sample:Not available


11). Gong SS et al. Mucosa repair mechanisms of Tong-Xie-Yao-Fang mediated by CRH-R2 in murine, dextran sulfate sodium-induced colitis. World J Gastroenterol 2018 Apr 28;24(16):1766-1778 (PubMed: 29713130)

12). Xiong K et al. Identification of HDAC9 as a viable therapeutic target for the treatment of gastric cancer. Exp Mol Med 2019 Aug 26;51(8):100 (PubMed: 31451695)

评论: Ki67 Antibody
Catalog No. : AF0198麻烦报个价。谢谢。南京良纬生物科技有限公司

Anonymous | 2016-04-13


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Catalog Number :

AF0198-BP
(Blocking peptide available as AF0198-BP)

Price/Size :

¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
100%
Bovine
100%
Dog
82%
Chicken
55%
Xenopus
45%
Horse
0%
Pig
0%
Zebrafish
0%
Sheep
0%
High similarity Medium similarity Low similarity No similarity
P46013 as Substrate
Site PTM Type Enzyme
K11 Ubiquitination
K46 Ubiquitination
K83 Ubiquitination
R93 Methylation
K107 Ubiquitination
S108 Phosphorylation
T109 Phosphorylation
K114 Ubiquitination
S125 Phosphorylation
S127 Phosphorylation
S128 Phosphorylation
S130 Phosphorylation
S131 Phosphorylation
K136 Ubiquitination
K145 Ubiquitination
K150 Ubiquitination
K160 Ubiquitination
K163 Sumoylation
K163 Ubiquitination
K172 Ubiquitination
S184 Phosphorylation
S185 Phosphorylation
K204 Sumoylation
K204 Ubiquitination
K210 Ubiquitination
K219 Methylation
K219 Ubiquitination
K231 Ubiquitination
K232 Ubiquitination
S235 Phosphorylation
K239 Ubiquitination
Y241 Phosphorylation
S243 Phosphorylation
K245 Sumoylation
K245 Ubiquitination
K251 Ubiquitination
K254 Ubiquitination
K263 Ubiquitination
S264 Phosphorylation
T268 Phosphorylation
K274 Ubiquitination
S296 Phosphorylation
S299 Phosphorylation
S308 Phosphorylation
K317 Ubiquitination
S325 Phosphorylation
T328 Phosphorylation
S330 Phosphorylation
K331 Ubiquitination
S336 Phosphorylation
Y340 Phosphorylation
K344 Ubiquitination
K346 Ubiquitination
T347 Phosphorylation
S352 Phosphorylation
S357 Phosphorylation P24941 (CDK2)
K360 Ubiquitination
K364 Ubiquitination
Y367 Phosphorylation
T368 Phosphorylation
S374 Phosphorylation Q96GD4 (AURKB)
K379 Acetylation
K379 Ubiquitination
K388 Acetylation
T389 Phosphorylation
T391 Phosphorylation
S396 Phosphorylation
T401 Phosphorylation
S411 Phosphorylation
T413 Phosphorylation
K414 Ubiquitination
K421 Ubiquitination
S425 Phosphorylation
K458 Ubiquitination
S460 Phosphorylation
K466 Acetylation
K502 Methylation
K502 Ubiquitination
S507 Phosphorylation
T525 Phosphorylation
K528 Ubiquitination
K537 Ubiquitination
S538 Phosphorylation Q96GD4 (AURKB)
T543 Phosphorylation
K548 Ubiquitination
K552 Sumoylation
K552 Ubiquitination
K560 Ubiquitination
S563 Phosphorylation
S565 Phosphorylation
S575 Phosphorylation
S579 Phosphorylation
S584 Phosphorylation
K587 Sumoylation
K587 Ubiquitination
T588 Phosphorylation
S592 Phosphorylation
S598 Phosphorylation
T601 Phosphorylation
S605 Phosphorylation
K608 Ubiquitination
T611 Phosphorylation
K615 Ubiquitination
K620 Ubiquitination
S621 Phosphorylation
K627 Ubiquitination
S634 Phosphorylation
K645 Ubiquitination
S648 Phosphorylation
S651 Phosphorylation
K659 Ubiquitination
S660 Phosphorylation
K666 Ubiquitination
K670 Ubiquitination
K675 Ubiquitination
T694 Phosphorylation
K696 Ubiquitination
K697 Ubiquitination
S704 Phosphorylation
S707 Phosphorylation
S713 Phosphorylation
T716 Phosphorylation
K721 Ubiquitination
K726 Ubiquitination
K745 Sumoylation
K745 Ubiquitination
K749 Sumoylation
K749 Ubiquitination
S753 Phosphorylation
K760 Ubiquitination
T761 Phosphorylation P06493 (CDK1)
K764 Ubiquitination
K818 Ubiquitination
S825 Phosphorylation
S827 Phosphorylation
T844 Phosphorylation
Y849 Phosphorylation
K850 Ubiquitination
S853 Phosphorylation
K857 Ubiquitination
T858 Phosphorylation
S859 Phosphorylation
K867 Ubiquitination
S878 Phosphorylation
K886 Ubiquitination
K892 Sumoylation
K892 Ubiquitination
K906 Ubiquitination
K910 Ubiquitination
K923 Ubiquitination
K933 Ubiquitination
K957 Ubiquitination
K968 Ubiquitination
S969 Phosphorylation
K977 Ubiquitination
T988 Phosphorylation
K993 Ubiquitination
K1004 Ubiquitination
T1017 Phosphorylation
K1022 Ubiquitination
K1026 Ubiquitination
S1028 Phosphorylation
K1031 Ubiquitination
K1035 Sumoylation
K1035 Ubiquitination
K1043 Ubiquitination
K1063 Acetylation
K1063 Ubiquitination
T1067 Phosphorylation
K1069 Sumoylation
S1071 Phosphorylation
K1073 Ubiquitination
T1091 Phosphorylation
K1093 Sumoylation
K1093 Ubiquitination
S1098 Phosphorylation
K1106 Ubiquitination
T1111 Phosphorylation
S1115 Phosphorylation
S1118 Phosphorylation
S1131 Phosphorylation
S1136 Phosphorylation
T1139 Phosphorylation
T1141 Phosphorylation
S1142 Phosphorylation
T1143 Phosphorylation
K1144 Ubiquitination
K1153 Sumoylation
K1153 Ubiquitination
K1165 Acetylation
K1165 Ubiquitination
T1167 Phosphorylation
S1169 Phosphorylation
K1172 Ubiquitination
T1176 Phosphorylation
K1178 Acetylation
K1185 Acetylation
K1188 Sumoylation
K1188 Ubiquitination
T1193 Phosphorylation
K1197 Ubiquitination
T1203 Phosphorylation
S1207 Phosphorylation
K1208 Acetylation
K1208 Ubiquitination
T1213 Phosphorylation
K1217 Ubiquitination
K1228 Ubiquitination
T1233 Phosphorylation
T1237 Phosphorylation
K1245 Ubiquitination
K1248 Ubiquitination
C1251 S-Nitrosylation
S1253 Phosphorylation
S1256 Phosphorylation
T1261 Phosphorylation
T1263 Phosphorylation
S1264 Phosphorylation
T1265 Phosphorylation
K1266 Ubiquitination
S1272 Phosphorylation
K1275 Ubiquitination
S1291 Phosphorylation
T1298 Phosphorylation
S1302 Phosphorylation
T1315 Phosphorylation
T1323 Phosphorylation
T1327 Phosphorylation
S1329 Phosphorylation
K1330 Acetylation
K1330 Ubiquitination
T1335 Phosphorylation
K1337 Ubiquitination
T1347 Phosphorylation
K1350 Ubiquitination
T1355 Phosphorylation
T1359 Phosphorylation
K1367 Ubiquitination
S1375 Phosphorylation
S1376 Phosphorylation
S1380 Phosphorylation
T1383 Phosphorylation
T1385 Phosphorylation
S1386 Phosphorylation
T1387 Phosphorylation
T1393 Phosphorylation
K1402 Ubiquitination
S1405 Phosphorylation
K1408 Acetylation
K1409 Ubiquitination
T1411 Phosphorylation
S1414 Phosphorylation
K1422 Ubiquitination
K1441 Ubiquitination
S1447 Phosphorylation
T1449 Phosphorylation
S1451 Phosphorylation
K1452 Acetylation
K1452 Ubiquitination
K1460 Ubiquitination
K1471 Ubiquitination
T1476 Phosphorylation
T1480 Phosphorylation
K1482 Acetylation
K1482 Ubiquitination
T1485 Phosphorylation
S1496 Phosphorylation
T1503 Phosphorylation
T1505 Phosphorylation
S1506 Phosphorylation
S1507 Phosphorylation
K1508 Ubiquitination
S1511 Phosphorylation
K1512 Methylation
K1512 Ubiquitination
S1514 Phosphorylation
K1517 Sumoylation
K1517 Ubiquitination
T1531 Phosphorylation
S1533 Phosphorylation
T1540 Phosphorylation
K1542 Ubiquitination
S1546 Phosphorylation
K1549 Ubiquitination
Y1552 Phosphorylation
T1557 Phosphorylation
T1565 Phosphorylation
T1569 Phosphorylation
S1571 Phosphorylation
K1572 Acetylation
K1572 Ubiquitination
T1577 Phosphorylation
K1581 Acetylation
K1581 Ubiquitination
K1592 Acetylation
K1592 Ubiquitination
T1597 Phosphorylation
T1601 Phosphorylation
S1604 Phosphorylation
K1612 Acetylation
S1618 Phosphorylation
S1628 Phosphorylation
S1629 Phosphorylation
S1636 Phosphorylation
K1639 Acetylation
K1639 Ubiquitination
K1643 Sumoylation
K1643 Ubiquitination
K1651 Ubiquitination
S1656 Phosphorylation
T1660 Phosphorylation
S1679 Phosphorylation
S1686 Phosphorylation
S1689 Phosphorylation
T1691 Phosphorylation
S1693 Phosphorylation
K1694 Ubiquitination
T1699 Phosphorylation
K1703 Sumoylation
K1703 Ubiquitination
S1704 Phosphorylation
T1719 Phosphorylation
S1721 Phosphorylation
K1724 Ubiquitination
S1726 Phosphorylation
K1734 Ubiquitination
S1736 Phosphorylation
Y1737 Phosphorylation
S1740 Phosphorylation
T1747 Phosphorylation
T1749 Phosphorylation
S1750 Phosphorylation
S1751 Phosphorylation
K1752 Ubiquitination
S1758 Phosphorylation
K1761 Ubiquitination
T1764 Phosphorylation O14757 (CHEK1)
T1775 Phosphorylation
S1777 Phosphorylation
T1784 Phosphorylation
K1793 Ubiquitination
T1797 Phosphorylation
T1801 Phosphorylation
K1805 Ubiquitination
S1815 Phosphorylation
K1825 Ubiquitination
T1841 Phosphorylation
T1844 Phosphorylation
T1848 Phosphorylation
T1849 Phosphorylation
K1852 Ubiquitination
T1853 Phosphorylation
K1860 Methylation
K1860 Ubiquitination
S1861 Phosphorylation
S1864 Phosphorylation
T1869 Phosphorylation
T1871 Phosphorylation
K1874 Ubiquitination
S1880 Phosphorylation
K1883 Sumoylation
K1883 Ubiquitination
K1895 Ubiquitination
T1897 Phosphorylation
S1899 Phosphorylation
K1902 Acetylation
K1902 Ubiquitination
T1906 Phosphorylation
K1915 Ubiquitination
T1919 Phosphorylation
T1923 Phosphorylation
K1927 Ubiquitination
S1937 Phosphorylation
K1938 Acetylation
K1938 Ubiquitination
T1943 Phosphorylation P06493 (CDK1)
K1949 Ubiquitination
K1958 Ubiquitination
T1963 Phosphorylation
T1967 Phosphorylation
S1970 Phosphorylation
S1980 Phosphorylation
K1982 Ubiquitination
S1983 Phosphorylation
T1991 Phosphorylation
S2002 Phosphorylation
K2005 Acetylation
K2005 Ubiquitination
K2009 Sumoylation
K2009 Ubiquitination
T2019 Phosphorylation
T2021 Phosphorylation
T2025 Phosphorylation
T2026 Phosphorylation
T2028 Phosphorylation
T2065 Phosphorylation
K2067 Sumoylation
K2067 Ubiquitination
S2072 Phosphorylation
K2080 Ubiquitination
T2085 Phosphorylation
T2089 Phosphorylation
S2105 Phosphorylation
S2110 Phosphorylation
T2113 Phosphorylation
T2115 Phosphorylation
S2116 Phosphorylation
T2123 Phosphorylation
S2135 Phosphorylation
K2138 Ubiquitination
K2167 Ubiquitination
S2173 Phosphorylation
T2175 Phosphorylation
K2178 Acetylation
K2178 Ubiquitination
K2187 Ubiquitination
K2198 Ubiquitination
T2203 Phosphorylation
T2207 Phosphorylation
T2211 Phosphorylation
T2212 Phosphorylation
T2217 Phosphorylation
S2223 Phosphorylation
T2231 Phosphorylation
T2233 Phosphorylation
K2236 Ubiquitination
S2239 Phosphorylation
K2240 Methylation
S2242 Phosphorylation
K2245 Ubiquitination
S2252 Phosphorylation
T2259 Phosphorylation
S2261 Phosphorylation
K2264 Acetylation
T2268 Phosphorylation
K2280 Acetylation
K2280 Sumoylation
T2285 Phosphorylation
K2289 Acetylation
K2289 Ubiquitination
S2299 Phosphorylation
K2300 Acetylation
K2300 Ubiquitination
T2305 Phosphorylation
K2309 Ubiquitination
K2320 Ubiquitination
T2325 Phosphorylation
T2328 Phosphorylation
K2330 Ubiquitination
T2332 Phosphorylation
T2333 Phosphorylation
T2337 Phosphorylation
K2343 Methylation
K2343 Ubiquitination
S2344 Phosphorylation
T2352 Phosphorylation
S2355 Phosphorylation
T2356 Phosphorylation
K2366 Sumoylation
K2366 Ubiquitination
T2380 Phosphorylation
S2382 Phosphorylation
T2389 Phosphorylation
S2395 Phosphorylation
K2398 Sumoylation
K2398 Ubiquitination
T2402 Phosphorylation
T2406 Phosphorylation
K2410 Ubiquitination
S2420 Phosphorylation
K2421 Acetylation
K2421 Ubiquitination
T2426 Phosphorylation
K2430 Sumoylation
K2430 Ubiquitination
K2441 Ubiquitination
T2446 Phosphorylation
T2450 Phosphorylation
S2453 Phosphorylation
T2455 Phosphorylation
T2460 Phosphorylation
S2463 Phosphorylation
S2466 Phosphorylation
S2471 Phosphorylation
K2473 Methylation
K2488 Ubiquitination
K2492 Sumoylation
K2500 Ubiquitination
T2502 Phosphorylation
T2504 Phosphorylation
S2505 Phosphorylation
T2508 Phosphorylation
T2509 Phosphorylation
T2516 Phosphorylation
K2520 Acetylation
K2523 Acetylation
S2528 Phosphorylation
K2530 Ubiquitination
S2538 Phosphorylation
S2542 Phosphorylation
K2587 Ubiquitination
S2588 Phosphorylation
T2594 Phosphorylation
T2598 Phosphorylation
T2600 Phosphorylation
K2601 Ubiquitination
K2613 Sumoylation
K2613 Ubiquitination
K2634 Ubiquitination
S2638 Phosphorylation
T2683 Phosphorylation
T2692 Phosphorylation
S2695 Phosphorylation
T2697 Phosphorylation
S2708 Phosphorylation
T2716 Phosphorylation
T2717 Phosphorylation
S2719 Phosphorylation
T2720 Phosphorylation
K2734 Sumoylation
T2749 Phosphorylation
T2750 Phosphorylation
K2767 Ubiquitination
S2769 Phosphorylation
T2773 Phosphorylation
T2781 Phosphorylation
S2783 Phosphorylation
S2793 Phosphorylation
S2814 Phosphorylation
T2816 Phosphorylation
S2827 Phosphorylation
S2828 Phosphorylation
T2834 Phosphorylation
T2836 Phosphorylation
S2837 Phosphorylation
S2838 Phosphorylation
K2852 Sumoylation
K2852 Ubiquitination
T2862 Phosphorylation
K2892 Ubiquitination
S2901 Phosphorylation
S2920 Phosphorylation
S2925 Phosphorylation
T2927 Phosphorylation
T2931 Phosphorylation
S2941 Phosphorylation
T2949 Phosphorylation
S2952 Phosphorylation
K2967 Ubiquitination
K2986 Acetylation
K2997 Acetylation
K2997 Methylation
T3007 Phosphorylation
T3009 Phosphorylation
S3041 Phosphorylation
S3042 Phosphorylation
S3067 Phosphorylation
S3082 Phosphorylation
K3087 Ubiquitination
T3127 Phosphorylation
S3128 Phosphorylation
K3180 Ubiquitination
S3197 Phosphorylation
T3213 Phosphorylation
S3216 Phosphorylation
K3217 Ubiquitination
S3218 Phosphorylation
T3223 Phosphorylation
S3249 Phosphorylation
S3253 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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