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  • 名 称:
    Paxillin 抗体
  • 货 号:
    AF6332
  • RRID:
    AB_2835188
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,ELISA(peptide)
  • 反 应:
    Human, Mouse, Rat
  • 预 测:
    Pig, Bovine, Sheep, Rabbit, Dog, Chicken
  • 蛋白号:
  • 分子量:
    68kD;
    65kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

FLJ16691; FLJ23042; Paired box protein Pax 1; PAX 1; PAX1; PAXI_HUMAN; Paxillin alpha; Paxillin; PXN; PXN protein;

应用:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human, Mouse, Rat

预测反应:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(91%)

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

特异性:

Paxillin Antibody detects endogenous levels of total Paxillin.

RRID:

AB_2835188
引用格式: Affinity Biosciences Cat# AF6332, RRID:AB_2835188.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human Paxillin, corresponding to a region within N-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PXN

Molecular Weight:

Observed Mol.Wt.: 68kD.
Predicted Mol.Wt.: 65kDa(Calculated)..

Subcellular Location:

Cytoplasm > cytoskeleton. Cell junction > focal adhesion.

Description:

PXN is a multi-domain cytoskeletal protein that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions.

Sequence:
MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC

研究背景

Function:

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Post-translational Modifications:

Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion. Cytoplasm>Cell cortex.
Note: Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Subunit Structure:

Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Interacts with SORBS1, PARVA and PARVB. Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent. Interacts with LIMA1; this complex stabilizes actin dynamics.

Similarity:

Belongs to the paxillin family.

研究领域

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration.(View pathway)

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Catalog Number :

AF6332-BP
(Blocking peptide available as AF6332-BP)

Price/Size :

¥2000/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Bovine
100%
Sheep
100%
Dog
100%
Chicken
91%
Rabbit
91%
Xenopus
73%
Horse
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
P49023 as Substrate
Site PTM Type Enzyme
M1 Acetylation
T29 Phosphorylation
Y31 Phosphorylation A0A059VC25 (FAK) , Q13882 (PTK6) , P00519 (ABL1) , Q05397 (PTK2)
S32 Phosphorylation
Y33 Phosphorylation
T35 Phosphorylation
T39 Phosphorylation
Y40 Phosphorylation P12931 (SRC)
S83 Phosphorylation
S84 Phosphorylation
S85 Phosphorylation Q16539 (MAPK14) , Q00535 (CDK5)
Y88 Phosphorylation P12931 (SRC)
S90 Phosphorylation
S95 Phosphorylation
S103 Phosphorylation
S106 Phosphorylation
C108 S-Nitrosylation
S109 Phosphorylation
Y118 Phosphorylation P00519 (ABL1) , Q05397 (PTK2) , Q13882 (PTK6) , A0A059VC25 (FAK)
S119 Phosphorylation
K123 Ubiquitination
K125 Ubiquitination
S126 Phosphorylation Q9UBE8 (NLK)
S130 Phosphorylation P27361 (MAPK3)
T132 Phosphorylation
S135 Phosphorylation
T136 Phosphorylation
S137 Phosphorylation
S140 Phosphorylation
S143 Phosphorylation
S178 Phosphorylation P45983 (MAPK8)
Y181 Phosphorylation
S188 Phosphorylation
S219 Phosphorylation
S230 Phosphorylation
S243 Phosphorylation
S244 Phosphorylation
T249 Phosphorylation
S250 Phosphorylation
T251 Phosphorylation
T255 Phosphorylation
S258 Phosphorylation Q13153 (PAK1)
S260 Phosphorylation
S261 Phosphorylation
T263 Phosphorylation
S272 Phosphorylation Q13177 (PAK2) , O96013 (PAK4) , Q13153 (PAK1)
S274 Phosphorylation Q13177 (PAK2)
K277 Ubiquitination
S302 Phosphorylation
S303 Phosphorylation
T318 Phosphorylation
S320 Phosphorylation
S321 Phosphorylation
S322 Phosphorylation
S332 Phosphorylation
S336 Phosphorylation
S340 Phosphorylation
K355 Ubiquitination
T371 Phosphorylation
Y409 Phosphorylation
S414 Phosphorylation
Y468 Phosphorylation
S533 Phosphorylation
K540 Ubiquitination
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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