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  • 名 称:
    JAK2 抗体
  • 货 号:
    AF6025
  • RRID:
    AB_2834958
  • 来 源:
    Rabbit
  • 应 用:
    WB,IF/ICC,ELISA(peptide)
  • 反 应:
    Human, Mouse, Rat
  • 预 测:
    Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
  • 蛋白号:
  • 分子量:
    125kD;
    131kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

JAK 2; JAK-2; JAK2; JAK2_HUMAN; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase); Janus kinase 2; JTK 10; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2;

应用:

WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human, Mouse, Rat

预测反应:

Pig(92%), Bovine(85%), Horse(92%), Sheep(85%), Rabbit(92%), Dog(92%), Chicken(85%)

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

特异性:

JAK2 Antibody detects endogenous levels of total JAK2.

RRID:

AB_2834958
引用格式: Affinity Biosciences Cat# AF6025, RRID:AB_2834958.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human JAK2, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

JAK2

Molecular Weight:

Observed Mol.Wt.: 125kD.
Predicted Mol.Wt.: 131kDa(Calculated)..

Subcellular Location:

Endomembrane system. Nucleus.

Tissue Specificity:

O60674 JAK2_HUMAN:
Ubiquitously expressed throughout most tissues.

Description:

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon.

Sequence:
MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG

研究背景

Function:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Post-translational Modifications:

Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.

Subcellular Location:

Endomembrane system>Peripheral membrane protein. Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Tissue Specificity:

Ubiquitously expressed throughout most tissues.

Subunit Structure:

Interacts with EPOR, LYN, SIRPA, SH2B1 and TEC (By similarity). Interacts with IL23R. Interacts with SKB1. Interacts with STAM2. Interacts with IFNGR2 (via intracellular domain). Interacts with LEPR (Isoform B) (By similarity). Interacts with HSP90AB1; promotes functional activation in a heat shock-dependent manner. Interacts with STRA6. Interacts with RHEX; this interaction occurs in a erythropoietin (EPO)-dependent manner.

Similarity:

Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1 (By similarity).

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

研究领域

Research Fields:

· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.(View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Endocrine system > Prolactin signaling pathway.(View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

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Catalog Number :

AF6025-BP
(Blocking peptide available as AF6025-BP)

Price/Size :

¥2000/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
92%
Horse
92%
Dog
92%
Rabbit
92%
Bovine
85%
Sheep
85%
Chicken
85%
Xenopus
54%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
O60674 as Substrate
Site PTM Type Enzyme
Y119 Phosphorylation O60674 (JAK2)
K167 Sumoylation
T174 Phosphorylation
Y201 Phosphorylation O60674 (JAK2)
Y206 Phosphorylation O60674 (JAK2)
K207 Ubiquitination
K212 Ubiquitination
Y221 Phosphorylation O60674 (JAK2)
K244 Ubiquitination
K269 Ubiquitination
Y317 Phosphorylation O60674 (JAK2)
S358 Phosphorylation
Y372 Phosphorylation
Y373 Phosphorylation
Y382 Phosphorylation
Y423 Phosphorylation
Y435 Phosphorylation
S473 Phosphorylation
S518 Phosphorylation
T522 Phosphorylation
S523 Phosphorylation P27361 (MAPK3) , O60674 (JAK2)
Y570 Phosphorylation O60674 (JAK2)
K581 Ubiquitination
K630 Sumoylation
Y637 Phosphorylation O60674 (JAK2)
K728 Ubiquitination
K762 Ubiquitination
Y790 Phosphorylation
Y813 Phosphorylation O60674 (JAK2)
Y868 Phosphorylation O60674 (JAK2)
K882 Ubiquitination
K883 Ubiquitination
K903 Ubiquitination
S904 Phosphorylation
K912 Sumoylation
K914 Sumoylation
Y931 Phosphorylation
Y934 Phosphorylation
S936 Phosphorylation
Y940 Phosphorylation
Y956 Phosphorylation
Y966 Phosphorylation O60674 (JAK2)
Y972 Phosphorylation O60674 (JAK2)
K991 Sumoylation
K999 Ubiquitination
Y1007 Phosphorylation P40189 (IL6ST) , P00519 (ABL1) , O60674 (JAK2)
Y1008 Phosphorylation O60674 (JAK2) , P40189 (IL6ST)
K1011 Sumoylation
K1011 Ubiquitination
K1069 Acetylation
O60674 as PTM Enzyme
Substrate Site Source
O14543 (SOCS3) Y204 Uniprot
O14543 (SOCS3) Y221 Uniprot
O14744 (PRMT5) Y297 Uniprot
O14744 (PRMT5) Y304 Uniprot
O14744 (PRMT5) Y307 Uniprot
O60674 (JAK2) Y119 Uniprot
O60674 (JAK2) Y201 Uniprot
O60674 (JAK2) Y206 Uniprot
O60674 (JAK2) Y221 Uniprot
O60674 (JAK2) Y317 Uniprot
O60674 (JAK2) S523 Uniprot
O60674 (JAK2) Y570 Uniprot
O60674 (JAK2) Y637 Uniprot
O60674 (JAK2) Y813 Uniprot
O60674 (JAK2) Y868 Uniprot
O60674 (JAK2) Y966 Uniprot
O60674 (JAK2) Y972 Uniprot
O60674 (JAK2) Y1007 Uniprot
O60674 (JAK2) Y1008 Uniprot
P00533-1 (EGFR) Y1069 Uniprot
P00533 (EGFR) Y1092 Uniprot
P04049-1 (RAF1) Y340 Uniprot
P04049-1 (RAF1) Y341 Uniprot
P10912 (GHR) Y332 Uniprot
P10912 (GHR) Y487 Uniprot
P16410 (CTLA4) Y201 Uniprot
P19235-1 (EPOR) Y368 Uniprot
P19235-1 (EPOR) Y426 Uniprot
P19235-1 (EPOR) Y454 Uniprot
P19235-1 (EPOR) Y456 Uniprot
P19235-1 (EPOR) Y468 Uniprot
P19235-1 (EPOR) Y485 Uniprot
P19235-1 (EPOR) Y489 Uniprot
P19235-1 (EPOR) Y504 Uniprot
P27361 (MAPK3) Y204 Uniprot
P28482 (MAPK1) Y187 Uniprot
P40763-2 (STAT3) Y704 Uniprot
P40763-3 (STAT3) Y705 Uniprot
P41597 (CCR2) Y139 Uniprot
P42224 (STAT1) Y701 Uniprot
P42229-1 (STAT5A) Y694 Uniprot
P42680 (TEC) Y519 Uniprot
P46527 (CDKN1B) Y88 Uniprot
P51692 (STAT5B) Y699 Uniprot
P62993 (GRB2) Y7 Uniprot
P62993 (GRB2) Y37 Uniprot
P62993 (GRB2) Y52 Uniprot
P62993 (GRB2) Y209 Uniprot
P78324-1 (SIRPA) Y496 Uniprot
P78347 (GTF2I) Y248 Uniprot
P84243 (H3F3B) Y42 Uniprot
Q13153 (PAK1) Y153 Uniprot
Q13153 (PAK1) Y201 Uniprot
Q13153 (PAK1) Y285 Uniprot
Q14765 (STAT4) Y693 Uniprot
Q15118 (PDK1) Y243 Uniprot
Q15910 (EZH2) Y641 Uniprot
Q92888 (ARHGEF1) Y738 Uniprot
Q99683 (MAP3K5) Y718 Uniprot
Q9UGK3 (STAP2) Y22 Uniprot
Q9UGK3-2 (STAP2) Y250 Uniprot
Q9UGK3 (STAP2) Y310 Uniprot
Q9UGK3 (STAP2) Y322 Uniprot
Q9UQC2 (GAB2) Y643 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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